Interleukin-1 receptor antagonist production in human and murine lens

M. C. Kennedy, Stephen Planck, J. C. Ansel, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

Abstract

Purpose: Interleukin-1 receptor antagonist (IL-1ra) can effectively reduce target cell response to interleukin-1 (IL-1) by competition for binding to the IL-1 type 1 receptor. We examined normal human and murine lenses as well as cultured lens epithelial cells for the expression of IL-1ra mRNA and the production of IL-1ra protein. Methods: Expression of IL-1ra mRNA was studied by reverse transcriptase polymerase chain reaction (RT-PCR) analysis of total RNA isolated from fresh human lens and cultured human lens epithelial cells. In situ RT-PCR technique was used to study the expression of IL-1ra mRNA by normal lenses. Quantitation of the IL-1ra protein produced by cultured lens epithelial cells was done by ELISA. Normal human lenses, obtained from eye bank donors, were examined by immunohistochemical staining of frozen sections. Results: Human lens epithelial cells showed constitutive IL-1ra protein and mRNA production. Stimulation by IL-1α of cultured cells upregulated IL-1ra mRNA levels and increased protein levels by 5-fold. IL-1ra specific in situ RT-PCR labeled all epithelial cells of normal murine lens. Immunohistochemical staining of the normal human lens showed positive staining thoughout the epithelium and in the lens fiber layer. Conclusion: IL-1ra is produced by the cells of human and murine lens epithelium. IL-1ra production by these cells is constitutive and can be upregulated by cytokines.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

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Interleukin-1 Receptors
Lenses
Interleukin 1 Receptor Antagonist Protein
Epithelial Cells
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Staining and Labeling
Interleukin-1
Interleukin-1 Type I Receptors
Epithelium
Eye Banks
Frozen Sections
Cultured Cells
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Ophthalmology

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Interleukin-1 receptor antagonist production in human and murine lens. / Kennedy, M. C.; Planck, Stephen; Ansel, J. C.; Rosenbaum, James (Jim).

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

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AU - Planck, Stephen

AU - Ansel, J. C.

AU - Rosenbaum, James (Jim)

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