Interleukin-1 enhances murine granulopoiesis in vivo

L. C. Stork, V. M. Peterson, C. H. Rundus, W. A. Robinson

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50 Scopus citations

Abstract

Interleukin-1 (IL-1), a monokine involved in host response to infection and inflammation, has recently been shown to stimulate production of granulocyte-monocyte colony-stimulating factors (CSFs) from a variety of cell types in vitro. The purpose of this study was to investigate the effects of human IL-1 on granulopoiesis in vivo. CF1 female mice were injected with a single dose of either highly purified human IL-1 or recombinant human IL-1α (rIL-1α). Heat-inactivated IL-1 or rIL-1α served as controls. Physiologic doses of the IL-1 preparations were initially established by evaluating neutrophil egress from bone marrow (BM). Significant peripheral neutrophilia developed 3 h after injection of 10 U (doubling units) purified IL-1, in association with decreased marrow neutrophils. Significant neutrophilia occurred 6 h after injection of 5 x 103 U (half-maximal units) rIL-1α. Serum colony-stimulating activity (CSA) and BM colony formation (CFU-GM) were subsequently measured in standard agar culture at various times following injection. A significant rise in CSA occurred between 3 and 6 after injection of purified IL-1, and a significant increase in BM CFU-GM developed 48 h after injection. Similar increases in CSA and CFU-GM occurred following injection of rIL-1α. These results suggest that IL-1 may play an important role in the regulation of granulopoiesis in vivo by enhancing the production of CSFs required for myeloid proliferation.

Original languageEnglish (US)
Pages (from-to)163-167
Number of pages5
JournalExperimental hematology
Volume16
Issue number2
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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    Stork, L. C., Peterson, V. M., Rundus, C. H., & Robinson, W. A. (1988). Interleukin-1 enhances murine granulopoiesis in vivo. Experimental hematology, 16(2), 163-167.