Interleukin-1 enhances murine granulopoiesis in vivo

Linda Stork, V. M. Peterson, C. H. Rundus, W. A. Robinson

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Interleukin-1 (IL-1), a monokine involved in host response to infection and inflammation, has recently been shown to stimulate production of granulocyte-monocyte colony-stimulating factors (CSFs) from a variety of cell types in vitro. The purpose of this study was to investigate the effects of human IL-1 on granulopoiesis in vivo. CF1 female mice were injected with a single dose of either highly purified human IL-1 or recombinant human IL-1α (rIL-1α). Heat-inactivated IL-1 or rIL-1α served as controls. Physiologic doses of the IL-1 preparations were initially established by evaluating neutrophil egress from bone marrow (BM). Significant peripheral neutrophilia developed 3 h after injection of 10 U (doubling units) purified IL-1, in association with decreased marrow neutrophils. Significant neutrophilia occurred 6 h after injection of 5 x 103 U (half-maximal units) rIL-1α. Serum colony-stimulating activity (CSA) and BM colony formation (CFU-GM) were subsequently measured in standard agar culture at various times following injection. A significant rise in CSA occurred between 3 and 6 after injection of purified IL-1, and a significant increase in BM CFU-GM developed 48 h after injection. Similar increases in CSA and CFU-GM occurred following injection of rIL-1α. These results suggest that IL-1 may play an important role in the regulation of granulopoiesis in vivo by enhancing the production of CSFs required for myeloid proliferation.

Original languageEnglish (US)
Pages (from-to)163-167
Number of pages5
JournalExperimental Hematology
Volume16
Issue number2
StatePublished - 1988
Externally publishedYes

Fingerprint

Interleukin-1
Granulocyte-Macrophage Progenitor Cells
Injections
Bone Marrow
Neutrophils
Monokines
Colony-Stimulating Factors
Granulocyte Colony-Stimulating Factor
Agar
Monocytes
Hot Temperature
Inflammation

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Stork, L., Peterson, V. M., Rundus, C. H., & Robinson, W. A. (1988). Interleukin-1 enhances murine granulopoiesis in vivo. Experimental Hematology, 16(2), 163-167.

Interleukin-1 enhances murine granulopoiesis in vivo. / Stork, Linda; Peterson, V. M.; Rundus, C. H.; Robinson, W. A.

In: Experimental Hematology, Vol. 16, No. 2, 1988, p. 163-167.

Research output: Contribution to journalArticle

Stork, L, Peterson, VM, Rundus, CH & Robinson, WA 1988, 'Interleukin-1 enhances murine granulopoiesis in vivo', Experimental Hematology, vol. 16, no. 2, pp. 163-167.
Stork L, Peterson VM, Rundus CH, Robinson WA. Interleukin-1 enhances murine granulopoiesis in vivo. Experimental Hematology. 1988;16(2):163-167.
Stork, Linda ; Peterson, V. M. ; Rundus, C. H. ; Robinson, W. A. / Interleukin-1 enhances murine granulopoiesis in vivo. In: Experimental Hematology. 1988 ; Vol. 16, No. 2. pp. 163-167.
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