Interleukin-1 accelertes murine granulocyte recovery following treatment with cyclophosphamide

L. Stork, L. Barczuk, M. Kissinger, W. Robinson

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38 Scopus citations

Abstract

This study investigated the effects of recombinant human interleukin-1 (rhIL-1α) on granulocyte recovery following treatment of mice with cyclophosphamide (CPM). CF1 mice were injected with 0.5 μg rhIL-1α or heat-inactivated rhIL-1α according to five different regimens, before and/or following 200 mg/kg CPM. Significant neutrophilia initially developed in treatment mice of all five regimens and accelerated granulocyte recovery occurred in treatment mice of four IL-1 regimens. Significant elevations in serum colony stimulating activity (CSA) occurred in treatment mice at a number of time points studied. In addition, marked increases in the percentage of maturing granulocyte precursors and the proportion of cells cycling in S and G2/M were observed in treatment marrow throughout the IL-1 regimen. Before granulocyte recovery, premature nuclear segmentation was noted in metamyelocytes of treatment marrow. Concomitant with granulocyte recovery, treatment marrow was significantly more cellular and contained more total CFU-GM, more CFU-GM in S phase, more cells in S and G2/M, and more mitotic figures than control marrow. Splenic myelopoiesis was also enhanced in treatment mice. These data suggest that IL-1 significantly hastens granulocyte recovery following treatment with CPM by enhancing both proliferation and maturation of myeloid precursors.

Original languageEnglish (US)
Pages (from-to)938-944
Number of pages7
JournalBlood
Volume73
Issue number4
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Stork, L., Barczuk, L., Kissinger, M., & Robinson, W. (1989). Interleukin-1 accelertes murine granulocyte recovery following treatment with cyclophosphamide. Blood, 73(4), 938-944.