Interleukin-1 accelertes murine granulocyte recovery following treatment with cyclophosphamide

Linda Stork, L. Barczuk, M. Kissinger, W. Robinson

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

This study investigated the effects of recombinant human interleukin-1 (rhIL-1α) on granulocyte recovery following treatment of mice with cyclophosphamide (CPM). CF1 mice were injected with 0.5 μg rhIL-1α or heat-inactivated rhIL-1α according to five different regimens, before and/or following 200 mg/kg CPM. Significant neutrophilia initially developed in treatment mice of all five regimens and accelerated granulocyte recovery occurred in treatment mice of four IL-1 regimens. Significant elevations in serum colony stimulating activity (CSA) occurred in treatment mice at a number of time points studied. In addition, marked increases in the percentage of maturing granulocyte precursors and the proportion of cells cycling in S and G2/M were observed in treatment marrow throughout the IL-1 regimen. Before granulocyte recovery, premature nuclear segmentation was noted in metamyelocytes of treatment marrow. Concomitant with granulocyte recovery, treatment marrow was significantly more cellular and contained more total CFU-GM, more CFU-GM in S phase, more cells in S and G2/M, and more mitotic figures than control marrow. Splenic myelopoiesis was also enhanced in treatment mice. These data suggest that IL-1 significantly hastens granulocyte recovery following treatment with CPM by enhancing both proliferation and maturation of myeloid precursors.

Original languageEnglish (US)
Pages (from-to)938-944
Number of pages7
JournalBlood
Volume73
Issue number4
StatePublished - 1989
Externally publishedYes

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Interleukin-1
Granulocytes
Cyclophosphamide
Recovery
Granulocyte-Macrophage Progenitor Cells
Bone Marrow
Granulocyte Precursor Cells
Myelopoiesis
S Phase
Hot Temperature
Serum

ASJC Scopus subject areas

  • Hematology

Cite this

Stork, L., Barczuk, L., Kissinger, M., & Robinson, W. (1989). Interleukin-1 accelertes murine granulocyte recovery following treatment with cyclophosphamide. Blood, 73(4), 938-944.

Interleukin-1 accelertes murine granulocyte recovery following treatment with cyclophosphamide. / Stork, Linda; Barczuk, L.; Kissinger, M.; Robinson, W.

In: Blood, Vol. 73, No. 4, 1989, p. 938-944.

Research output: Contribution to journalArticle

Stork, L, Barczuk, L, Kissinger, M & Robinson, W 1989, 'Interleukin-1 accelertes murine granulocyte recovery following treatment with cyclophosphamide', Blood, vol. 73, no. 4, pp. 938-944.
Stork, Linda ; Barczuk, L. ; Kissinger, M. ; Robinson, W. / Interleukin-1 accelertes murine granulocyte recovery following treatment with cyclophosphamide. In: Blood. 1989 ; Vol. 73, No. 4. pp. 938-944.
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