Interferon gene transfer by a hepatitis B virus vector efficiently suppresses wild-type virus infection

Ulrike Protzer, Michael Nassal, Pei-Wen Chiang, Michael Kirschfink, Heinz Schaller

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Hepatitis B viruses specifically target the liver, where they efficiently infect quiescent hepatocytes. Here we show that human and avian hepatitis B viruses can be converted into vectors for liver-directed gene transfer. These vectors allow hepatocyte-specific expression of a green fluorescent protein in vitro and in vivo. Moreover, when used to transduce a type I interferon gene, expression of interferon efficiently suppresses wild- type virus replication in the duck model of hepatitis B virus infection. These data suggest local cytokine production after hepatitis-B-virus-mediated gene transfer as a promising concept for the treatment of acquired liver diseases, including chronic hepatitis B.

Original languageEnglish (US)
Pages (from-to)10818-10823
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number19
DOIs
StatePublished - Sep 14 1999
Externally publishedYes

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Virus Diseases
Hepatitis B virus
Interferons
Hepatocytes
Duck Hepatitis B Viruses
Genes
Interferon Type I
Liver
Chronic Hepatitis B
Virus Replication
Green Fluorescent Proteins
Liver Diseases
Cytokines
Gene Expression

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Interferon gene transfer by a hepatitis B virus vector efficiently suppresses wild-type virus infection. / Protzer, Ulrike; Nassal, Michael; Chiang, Pei-Wen; Kirschfink, Michael; Schaller, Heinz.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 19, 14.09.1999, p. 10818-10823.

Research output: Contribution to journalArticle

Protzer, Ulrike ; Nassal, Michael ; Chiang, Pei-Wen ; Kirschfink, Michael ; Schaller, Heinz. / Interferon gene transfer by a hepatitis B virus vector efficiently suppresses wild-type virus infection. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 19. pp. 10818-10823.
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