Interferon-Gamma test for the detection of Mycobacterium tuberculosis complex infection in Macaca mulatta and other non-human primates

Jo Ann L. Yee, Kamm Prongay, Brodie Miles, Jeremy Smedley, Scott Hansen, Michael Axthelm, Amir Ardeshir, Koen K.A. Van Rompay, Gregory Timmel, Jeffrey A. Roberts

Research output: Contribution to journalArticle

Abstract

We have formatted an assay to detect Mycobacterium tuberculosis complex infections of non-human primates. Commercially available reagents were used to elicit a specific immune response that was measured by interferon-gamma release. Initial evaluation using blood samples from Rhesus macaques experimentally infected with M tuberculosis distinguished infected versus uninfected animals.

Original languageEnglish (US)
JournalJournal of medical primatology
DOIs
StatePublished - Jan 1 2019

Fingerprint

Mycobacterium tuberculosis complex
Macaca mulatta
interferon-gamma
Mycobacterium tuberculosis
Primates
Interferon-gamma
immune response
blood
assays
Infection
infection
animals
testing
sampling

Keywords

  • cell-mediated immune response (CMI)
  • interferon-gamma release assay (IGRA)
  • purified protein derivative—tuberculin (PPD)

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

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abstract = "We have formatted an assay to detect Mycobacterium tuberculosis complex infections of non-human primates. Commercially available reagents were used to elicit a specific immune response that was measured by interferon-gamma release. Initial evaluation using blood samples from Rhesus macaques experimentally infected with M tuberculosis distinguished infected versus uninfected animals.",
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author = "Yee, {Jo Ann L.} and Kamm Prongay and Brodie Miles and Jeremy Smedley and Scott Hansen and Michael Axthelm and Amir Ardeshir and {Van Rompay}, {Koen K.A.} and Gregory Timmel and Roberts, {Jeffrey A.}",
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AU - Yee, Jo Ann L.

AU - Prongay, Kamm

AU - Miles, Brodie

AU - Smedley, Jeremy

AU - Hansen, Scott

AU - Axthelm, Michael

AU - Ardeshir, Amir

AU - Van Rompay, Koen K.A.

AU - Timmel, Gregory

AU - Roberts, Jeffrey A.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - We have formatted an assay to detect Mycobacterium tuberculosis complex infections of non-human primates. Commercially available reagents were used to elicit a specific immune response that was measured by interferon-gamma release. Initial evaluation using blood samples from Rhesus macaques experimentally infected with M tuberculosis distinguished infected versus uninfected animals.

AB - We have formatted an assay to detect Mycobacterium tuberculosis complex infections of non-human primates. Commercially available reagents were used to elicit a specific immune response that was measured by interferon-gamma release. Initial evaluation using blood samples from Rhesus macaques experimentally infected with M tuberculosis distinguished infected versus uninfected animals.

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KW - interferon-gamma release assay (IGRA)

KW - purified protein derivative—tuberculin (PPD)

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