Interferon and phorbol esters down‐regulate slgM expression by independent pathways

Frederick M. Schaffer, Stephen H. Benedict, Dieter Petsche, Alan Lau, Bryan R.G. Williams, Gordon Mills, Erwin W. Gelfand

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We studied the effects of recombinant, interferon, 12‐O‐tetradecanoylphorbol13‐acetate (TPA), and phorbol 12,13 dibutyrate (PDB) on surface immunoglobulin expression by Daudi cells. Incubation of cells with recombinant alpha2interferon (IFN‐α2) caused a 2.5‐fold (60%) decrease in slgM expression as measured by relative fluorescence index (RFI) using a flow cytometer. This decrease in slgM expression was independent of inhibitory effects on proliferation and cell cycle progression. TPA or PDB also caused a threefold (67%) decrease in slgM expression, while enhancing proliferation and cell cycle progression. Coincubation of cells with IFN‐α2 or TPA decreased slgM expression by more than fourfold (>75%), which was greater than the decrease induced by the optimal concentration of either agent alone. Molecular studies demonstrated that the treatment of cells with IFN‐α2 or TPA decreased the steady‐state levels of mRNA for the heavy chain of IgM (cμ), suggesting that down‐regulation of slgM occurred at a pretranslational level. Activation of the cell membrane sodium/proton antiport did not play an integral role in the IFN‐α2 or phorbol‐ester‐induced pathway of slgM down‐regulation. Whereas IFN‐α2 induced an increase in the activity of 2′,5′‐oligoadenylate (2–5A) synthetase, the addition of TPA to IFN‐α2 caused a significant decrease in the activity of this enzyme. Although IFN‐α2 and TPA exhibited additive effects on slgM expression, they had opposing effects on cell proliferation, cell cycle progression, and induction of 2–5A synthetase activity, suggesting that these agents down‐regulate slgM expression through independent pathways.

Original languageEnglish (US)
Pages (from-to)245-252
Number of pages8
JournalJournal of Cellular Physiology
Volume134
Issue number2
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Phorbol Esters
Interferons
Down-Regulation
Cells
Phorbol 12,13-Dibutyrate
Cell Cycle
Ligases
B-Cell Antigen Receptors
Ion Transport
Immunoglobulin M
Cell proliferation
Protons
Cell membranes
Fluorescence
Sodium
Cell Proliferation
Cell Membrane
Messenger RNA
Chemical activation
Enzymes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Schaffer, F. M., Benedict, S. H., Petsche, D., Lau, A., Williams, B. R. G., Mills, G., & Gelfand, E. W. (1988). Interferon and phorbol esters down‐regulate slgM expression by independent pathways. Journal of Cellular Physiology, 134(2), 245-252. https://doi.org/10.1002/jcp.1041340210

Interferon and phorbol esters down‐regulate slgM expression by independent pathways. / Schaffer, Frederick M.; Benedict, Stephen H.; Petsche, Dieter; Lau, Alan; Williams, Bryan R.G.; Mills, Gordon; Gelfand, Erwin W.

In: Journal of Cellular Physiology, Vol. 134, No. 2, 01.01.1988, p. 245-252.

Research output: Contribution to journalArticle

Schaffer, FM, Benedict, SH, Petsche, D, Lau, A, Williams, BRG, Mills, G & Gelfand, EW 1988, 'Interferon and phorbol esters down‐regulate slgM expression by independent pathways', Journal of Cellular Physiology, vol. 134, no. 2, pp. 245-252. https://doi.org/10.1002/jcp.1041340210
Schaffer, Frederick M. ; Benedict, Stephen H. ; Petsche, Dieter ; Lau, Alan ; Williams, Bryan R.G. ; Mills, Gordon ; Gelfand, Erwin W. / Interferon and phorbol esters down‐regulate slgM expression by independent pathways. In: Journal of Cellular Physiology. 1988 ; Vol. 134, No. 2. pp. 245-252.
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