Interferon-γ inhibits in vitro mobilization of eosinophils by interleukin-5

Choon Sik Park, Nam Choi Eun, Sun Kim Jung, Sung Choi Yun, Youn Rhim Tai, Soo Chang Hun, Yup Chung Il

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Background: Th2 cytokines play pivotal roles in allergic inflammation, including eosinophilia, and their actions are antagonized by Th1 cytokines, conferring them therapeutic potential. Methods: In this study, we examined the ability of a number of cytokines to suppress the activation of eosinophils that function as effector cells for allergic airway diseases. Results: Interleukin (IL)-5, IL-6, and tumor necrosis factor (TNF) induced an eosinophil shape change, whereas interferon (IFN)-γ significantly inhibited the shape change. Other cytokines, including IL-1β, IL-4, IL-10 and IL-13, had little or only slightly enhancing or reducing effects on the shape change. We further analyzed the IFN-γ effect, showing that pretreatment with IFN-γ strongly suppressed IL-5-induced eosinophil shape change, and cycloheximide (CHX) abrogated the suppression by IFN-γ, suggesting that new protein synthesis is required for the inhibitory effect by this cytokine. In agreement with these results, IFN-γ blocked the eosinophil migration and ERK phophorylation induced by IL-5, and the addition of CHX restored eosinophil chemotaxis. Conclusions: Collectively, IFN-γ may attenuate eosinophilic inflammation by directly negating eosinophil mobilization.

Original languageEnglish (US)
Pages (from-to)295-302
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume136
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

Keywords

  • Asthma
  • Eosinophils
  • Interferon-γ
  • Interleukin-5
  • Interleukin-6
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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