The distribution of Ehrlich ascites tumor cells in the cell cycle was studied by flow cytometry following treatment with α-methyl ornithine (αMO) or methylglyoxal-bis(guanylhydrazone) (MeGAG). αMO and MeGAG are potent inhibitors of ornithine decarboxylase (putrescine synthesis) and S-adenosyl-methionine decarboxylase (spermidine and spermine synthesis), respectively. The data show that these polyamine synthesis inhibitors produce significant cell cycle perturbations resulting in an accumulation of cells in S and G2. This fact suggests that a normal polyamine complement is essential for the progression through these phases of the cell cycle.
ASJC Scopus subject areas
- Cell Biology