Intercellular signaling as visualized by endogenous calcium-dependent bioluminescence

Paul Brehm, James Lechleiter, Stephen Smith, Kathleen Dunlap

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Bioluminescence in the hydrozoan coelenterate Obelia results from calcium activation of a photoprotein contained in light-emitting cells (photocytes) scattered in the animal's endoderm. The influx of calcium into non-luminescent endodermal cells through conventional voltage-dependent calcium channels is required for the excitation-luminescence coupling. Our results suggest that the subsequent diffusion of this calcium, via gap junctions, into the neighboring photocytes triggers a localized luminescence response. Following intense stimulation, the local rise in calcium elicits a secondary wave of luminescence that is supported by a voltage-independent calcium permeability mechanism in the photocyte plasma membrane. These two mechanisms for elevating internal calcium in light-emitting cells can account for the spatial and temporal features of intra-cellular luminescence in Obelia.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalNeuron
Volume3
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

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Luminescence
Calcium
Luminescent Proteins
Light
Endoderm
Gap Junctions
Calcium Channels
Permeability
Cell Membrane

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Intercellular signaling as visualized by endogenous calcium-dependent bioluminescence. / Brehm, Paul; Lechleiter, James; Smith, Stephen; Dunlap, Kathleen.

In: Neuron, Vol. 3, No. 2, 1989, p. 191-198.

Research output: Contribution to journalArticle

Brehm, Paul ; Lechleiter, James ; Smith, Stephen ; Dunlap, Kathleen. / Intercellular signaling as visualized by endogenous calcium-dependent bioluminescence. In: Neuron. 1989 ; Vol. 3, No. 2. pp. 191-198.
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