Interactions of the ectodomain of HFE with the transferrin receptor are critical for iron homeostasis in cells

Cindy N. Roy, Eric J. Carlson, Emily L. Anderson, Alivelu Basava, Steven M. Starnes, John N. Feder, Caroline Enns

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Expression of wild type HFE reduces the ferritin levels of cells in culture. In this report we demonstrate that the predominant hereditary hemochromatosis mutation, C282Y2 HFE, does not reduce ferritin expression. However, the second mutation, H63D HFE, reduces ferritin expression to a level indistinguishable from cells expressing wild type HFE. Further, two HFE cytoplasmic domain mutations engineered to disrupt potential signal transduction, S335M and Y342C, were functionally indistinguishable from wild type HFE in this assay, as was soluble HFE. These results implicate a role for the interaction of HFE with the transferrin receptor in lowering cellular ferritin levels. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)271-274
Number of pages4
JournalFEBS Letters
Volume484
Issue number3
DOIs
StatePublished - Nov 10 2000

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Transferrin Receptors
Ferritins
Homeostasis
Iron
Mutation
Signal transduction
Hemochromatosis
Assays
Signal Transduction
Cell Culture Techniques
Cells

Keywords

  • Ferritin
  • Hemochromatosis
  • HFE
  • Iron metabolism
  • Transferrin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Interactions of the ectodomain of HFE with the transferrin receptor are critical for iron homeostasis in cells. / Roy, Cindy N.; Carlson, Eric J.; Anderson, Emily L.; Basava, Alivelu; Starnes, Steven M.; Feder, John N.; Enns, Caroline.

In: FEBS Letters, Vol. 484, No. 3, 10.11.2000, p. 271-274.

Research output: Contribution to journalArticle

Roy, Cindy N. ; Carlson, Eric J. ; Anderson, Emily L. ; Basava, Alivelu ; Starnes, Steven M. ; Feder, John N. ; Enns, Caroline. / Interactions of the ectodomain of HFE with the transferrin receptor are critical for iron homeostasis in cells. In: FEBS Letters. 2000 ; Vol. 484, No. 3. pp. 271-274.
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