Interactions of Eosinophils with Nerves

Matthew G. Drake, Becky J. Proskocil, Zhenying Nie

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Eosinophils affect nerve structure and function in organs such as lungs and skin, which contributes to disease pathogenesis. We have developed methods for culturing primary sensory and parasympathetic neurons in multiple species and have refined these techniques for coculture with eosinophils. Eosinophil-nerve coculture has been an essential tool for testing interactions between these cell types. Here we describe methods for coculturing primary parasympathetic ganglia, vagal sensory nerves, and dorsal root sensory nerves with eosinophils.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages161-181
Number of pages21
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2241
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Coculture
  • Dorsal root ganglia
  • Eosinophil
  • Nerves
  • Parasympathetic ganglia
  • Primary cell culture
  • Sensory Ganglia
  • Vagal ganglia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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