Interactions between Activating Signal Cointegrator-2 and the Tumor Suppressor Retinoblastoma in Androgen Receptor Transactivation

Young Hwa Goo, Soon Young Na, Hao Zhang, Jianming Xu, SunHwa Hong, JaeHun Cheong, Soo-Kyung Lee, Jae Lee

Research output: Contribution to journalArticle

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Abstract

Activating signal cointegrator-2 (ASC-2), a cancer-amplified transcription coactivator of nuclear receptors and numerous other transcription factors, was previously shown to contain two LXXLL motifs, each of which interacts with a distinct set of nuclear receptors. In this work, we showed that ASC-2 has an indirect, separate binding site for androgen receptor (AR). Interestingly, this region overlapped with the direct interaction interfaces with the tumor suppressor retinoblastoma (Rb). Although ASC-2 alone stimulated AR transactivation in cotransfections of HeLa cells, ectopic expression of Rb effected ASC-2 to act as a transcription coactivator of AR in Rb-null Saos2 cells. These results, along with the previous report in which AR was shown to directly interact with Rb (Yeh, S., Miyamoto, H., Nishimura, K., Kang, H., Ludlow, J., Hsiao, P., Wang, C., Su, C., and Chang C. (1998) Biochem. Biophys. Res. Commun. 248, 361-367), suggest that the AR-ASC-2 interactions in vivo may involve Rb. Thus, ASC-2 appears to contain at least three distinct nuclear receptor interaction domains.

Original languageEnglish (US)
Pages (from-to)7131-7135
Number of pages5
JournalJournal of Biological Chemistry
Volume279
Issue number8
DOIs
StatePublished - Feb 20 2004
Externally publishedYes

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Retinoblastoma
Androgen Receptors
Transcriptional Activation
Tumors
Transcription
Neoplasms
Cytoplasmic and Nuclear Receptors
Nuclear Receptor Coactivators
Null Lymphocytes
HeLa Cells
Transcription Factors
Binding Sites

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interactions between Activating Signal Cointegrator-2 and the Tumor Suppressor Retinoblastoma in Androgen Receptor Transactivation. / Goo, Young Hwa; Na, Soon Young; Zhang, Hao; Xu, Jianming; Hong, SunHwa; Cheong, JaeHun; Lee, Soo-Kyung; Lee, Jae.

In: Journal of Biological Chemistry, Vol. 279, No. 8, 20.02.2004, p. 7131-7135.

Research output: Contribution to journalArticle

Goo, Young Hwa ; Na, Soon Young ; Zhang, Hao ; Xu, Jianming ; Hong, SunHwa ; Cheong, JaeHun ; Lee, Soo-Kyung ; Lee, Jae. / Interactions between Activating Signal Cointegrator-2 and the Tumor Suppressor Retinoblastoma in Androgen Receptor Transactivation. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 8. pp. 7131-7135.
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