Interaction of thyroid-hormone receptor with a conserved transcriptional mediator

Jae Lee, Fergus Ryan, Jonathan C. Swaffield, Stephen A. Johnston, David D. Moore

Research output: Contribution to journalArticle

380 Citations (Scopus)

Abstract

THE thyroid-hormone receptors are hormone-dependent transcription factors that control expression of many target genes1,2. This regulation is presumably a consequence of hormone-dependent contacts between the receptors and the basal transcription machinery3. We used the yeast two-hybrid system 4,5 to identify a candidate human transcriptional mediator that interacts with both the thyroid-hormone receptor and the retinoid-X receptor in a ligand-dependent fashion. This protein, Tripl (for thyroid-hormone-receptor interacting protein), shares striking sequence conservation with the yeast transcriptional mediator Sugl (refs 6, 7). Here we show that Tripl can functionally substitute for Sugl in yeast, and that both proteins interact in vitro with the thyroid-hormone receptor, and with the transcriptional activation domains of yeast GAL4 and of herpes virus VP16.

Original languageEnglish (US)
Pages (from-to)91-94
Number of pages4
JournalNature
Volume374
Issue number6517
StatePublished - 1995
Externally publishedYes

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Thyroid Hormone Receptors
Yeasts
Hormones
Retinoid X Receptors
Two-Hybrid System Techniques
Fungal Proteins
Transcriptional Activation
Transcription Factors
Ligands
Viruses
Proteins

ASJC Scopus subject areas

  • General

Cite this

Lee, J., Ryan, F., Swaffield, J. C., Johnston, S. A., & Moore, D. D. (1995). Interaction of thyroid-hormone receptor with a conserved transcriptional mediator. Nature, 374(6517), 91-94.

Interaction of thyroid-hormone receptor with a conserved transcriptional mediator. / Lee, Jae; Ryan, Fergus; Swaffield, Jonathan C.; Johnston, Stephen A.; Moore, David D.

In: Nature, Vol. 374, No. 6517, 1995, p. 91-94.

Research output: Contribution to journalArticle

Lee, J, Ryan, F, Swaffield, JC, Johnston, SA & Moore, DD 1995, 'Interaction of thyroid-hormone receptor with a conserved transcriptional mediator', Nature, vol. 374, no. 6517, pp. 91-94.
Lee J, Ryan F, Swaffield JC, Johnston SA, Moore DD. Interaction of thyroid-hormone receptor with a conserved transcriptional mediator. Nature. 1995;374(6517):91-94.
Lee, Jae ; Ryan, Fergus ; Swaffield, Jonathan C. ; Johnston, Stephen A. ; Moore, David D. / Interaction of thyroid-hormone receptor with a conserved transcriptional mediator. In: Nature. 1995 ; Vol. 374, No. 6517. pp. 91-94.
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