Interaction of MHC class I molecules with the transporter associated with antigen processing

Woong Kyung Suh, Myrna F. Cohen-Doyle, Klaus Fruh, Kena Wang, Per A. Peterson, David B. Williams

Research output: Contribution to journalArticle

262 Scopus citations

Abstract

The transporter associated with antigen processing (TAP) delivers cytosolic peptides into the endoplasmic reticulum (ER) where they bind to nascent class I histocompatibility molecules. Class I-peptide complexes are then displayed at the cell surface for recognition by cytotoxic T lymphocytes. Immunoprecipitation of either TAP or class I molecules revealed an association between the transporter and diverse class I products. TAP bound preferentially to heterodimers of the class I heavy chain and β2-microglobulin, and the complex subsequently dissociated in parallel with transport of class I molecules from the ER to the Golgi apparatus. The TAP-class I complexes could also be dissociated in vitro by the addition of class I-binding peptides. The association of class I molecules with TAP likely promotes efficient capture of peptides before their exposure to the lumen of the ER.

Original languageEnglish (US)
Pages (from-to)1322-1326
Number of pages5
JournalScience
Volume264
Issue number5163
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • General

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    Suh, W. K., Cohen-Doyle, M. F., Fruh, K., Wang, K., Peterson, P. A., & Williams, D. B. (1994). Interaction of MHC class I molecules with the transporter associated with antigen processing. Science, 264(5163), 1322-1326. https://doi.org/10.1126/science.8191286