Interaction of IGF-binding protein-related protein 1 with a novel protein, neuroendocrine differentiation factor, results in neuroendocrine differentiation of prostate cancer cells

Elizabeth M. Wilson, Youngman Oh, Vivian Hwa, Ronald (Ron) Rosenfeld

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Neuroendocrine cells have been implicated in many cancers, including small cell lung, cervical, breast, and prostate carcinomas. The increase in neuroendocrine cell number in prostate cancer has been reported to correlate with poor prognosis, progressive tumors, and androgen insensitivity. The mechanisms involved in this differentiation remain unknown. IGF-binding protein-related protein 1 is a member of the IGF-binding protein superfamily and has recently been shown to exhibit differentiation and tumor suppression activity in prostate cancer cell lines stably overexpressing IGF-binding protein-related protein 1. From a yeast two-hybrid screen, a novel IGF-binding protein-related protein 1-interacting protein was identified. Immunocytochemical techniques indicate that this protein, 25.1, and intracellular IGF-binding protein-related protein 1 colocalize in the nucleus. When 25.1 is transiently expressed in a stable prostate cancer cell line overexpressing IGF-binding protein-related protein 1, cells assume a neuritic-like morphology with long dendritic-like processes and express the neuroendocrine markers chromogranin A and neuron-specific enolase. We propose that 25.1 (neuroendocrine differentiation factor) together with IGF-binding protein-related protein 1 can induce neuroendocrine cell differentiation in prostate cancer cells.

Original languageEnglish (US)
Pages (from-to)4504-4511
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume86
Issue number9
DOIs
StatePublished - 2001

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Insulin-Like Growth Factor Binding Proteins
Prostatic Neoplasms
Cells
Neuroendocrine Cells
Proteins
Tumors
Cell Line
Chromogranin A
Phosphopyruvate Hydratase
Small Cell Lung Carcinoma
Yeast
Androgens
Prostate
Cell Differentiation
Neoplasms
Cell Count
Yeasts
Breast Neoplasms

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Interaction of IGF-binding protein-related protein 1 with a novel protein, neuroendocrine differentiation factor, results in neuroendocrine differentiation of prostate cancer cells. / Wilson, Elizabeth M.; Oh, Youngman; Hwa, Vivian; Rosenfeld, Ronald (Ron).

In: Journal of Clinical Endocrinology and Metabolism, Vol. 86, No. 9, 2001, p. 4504-4511.

Research output: Contribution to journalArticle

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