Interaction between NF-κB- and serum response factor-binding elements activates an interleukin-2 receptor α-chain enhancer specifically in T lymphocytes

A. A. Kuang, K. D. Novak, S. M. Kang, K. Bruhn, M. J. Lenardo

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We find that a short enhancer element containing the NF-κB binding site from the interleukin-2 receptor α-chain gene (IL-2Rα) is preferentially activated in T cells. The IL-2Rα enhancer binds NF-κB poorly and is only weakly activated by the NF-κB site alone. Serum response factor (SRF) binds to a site adjacent to the NF-κB site in the IL-2R enhancer, and both sites together have strong transcriptional activity specifically in T cells. Surprisingly, the levels of SRF constitutively expressed in T cells are consistently higher than in other cell types. Overexpression of SRF in B cells causes the IL-2R enhancer to function as well as it does in T cells, suggesting that the high level of SRF binding in T cells is functionally important.

Original languageEnglish (US)
Pages (from-to)2536-2545
Number of pages10
JournalMolecular and cellular biology
Volume13
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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