Interaction between NF-κB- and serum response factor-binding elements activates an interleukin-2 receptor α-chain enhancer specifically in T lymphocytes

Anna Kuang, Kristine D. Novak, Sang Mo Kang, Kevin Bruhn, Michael J. Lenardo

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We find that a short enhancer element containing the NF-κB binding site from the interleukin-2 receptor α-chain gene (IL-2Rα) is preferentially activated in T cells. The IL-2Rα enhancer binds NF-κB poorly and is only weakly activated by the NF-κB site alone. Serum response factor (SRF) binds to a site adjacent to the NF-κB site in the IL-2R enhancer, and both sites together have strong transcriptional activity specifically in T cells. Surprisingly, the levels of SRF constitutively expressed in T cells are consistently higher than in other cell types. Overexpression of SRF in B cells causes the IL-2R enhancer to function as well as it does in T cells, suggesting that the high level of SRF binding in T cells is functionally important.

Original languageEnglish (US)
Pages (from-to)2536-2545
Number of pages10
JournalMolecular and Cellular Biology
Volume13
Issue number4
StatePublished - Apr 1993
Externally publishedYes

Fingerprint

Serum Response Factor
Interleukin-2 Receptors
T-Lymphocytes
Genes
B-Lymphocytes
Binding Sites

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Interaction between NF-κB- and serum response factor-binding elements activates an interleukin-2 receptor α-chain enhancer specifically in T lymphocytes. / Kuang, Anna; Novak, Kristine D.; Kang, Sang Mo; Bruhn, Kevin; Lenardo, Michael J.

In: Molecular and Cellular Biology, Vol. 13, No. 4, 04.1993, p. 2536-2545.

Research output: Contribution to journalArticle

Kuang, Anna ; Novak, Kristine D. ; Kang, Sang Mo ; Bruhn, Kevin ; Lenardo, Michael J. / Interaction between NF-κB- and serum response factor-binding elements activates an interleukin-2 receptor α-chain enhancer specifically in T lymphocytes. In: Molecular and Cellular Biology. 1993 ; Vol. 13, No. 4. pp. 2536-2545.
@article{d8dfba711b4343cfb98d2dbcb417b3e3,
title = "Interaction between NF-κB- and serum response factor-binding elements activates an interleukin-2 receptor α-chain enhancer specifically in T lymphocytes",
abstract = "We find that a short enhancer element containing the NF-κB binding site from the interleukin-2 receptor α-chain gene (IL-2Rα) is preferentially activated in T cells. The IL-2Rα enhancer binds NF-κB poorly and is only weakly activated by the NF-κB site alone. Serum response factor (SRF) binds to a site adjacent to the NF-κB site in the IL-2R enhancer, and both sites together have strong transcriptional activity specifically in T cells. Surprisingly, the levels of SRF constitutively expressed in T cells are consistently higher than in other cell types. Overexpression of SRF in B cells causes the IL-2R enhancer to function as well as it does in T cells, suggesting that the high level of SRF binding in T cells is functionally important.",
author = "Anna Kuang and Novak, {Kristine D.} and Kang, {Sang Mo} and Kevin Bruhn and Lenardo, {Michael J.}",
year = "1993",
month = "4",
language = "English (US)",
volume = "13",
pages = "2536--2545",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Interaction between NF-κB- and serum response factor-binding elements activates an interleukin-2 receptor α-chain enhancer specifically in T lymphocytes

AU - Kuang, Anna

AU - Novak, Kristine D.

AU - Kang, Sang Mo

AU - Bruhn, Kevin

AU - Lenardo, Michael J.

PY - 1993/4

Y1 - 1993/4

N2 - We find that a short enhancer element containing the NF-κB binding site from the interleukin-2 receptor α-chain gene (IL-2Rα) is preferentially activated in T cells. The IL-2Rα enhancer binds NF-κB poorly and is only weakly activated by the NF-κB site alone. Serum response factor (SRF) binds to a site adjacent to the NF-κB site in the IL-2R enhancer, and both sites together have strong transcriptional activity specifically in T cells. Surprisingly, the levels of SRF constitutively expressed in T cells are consistently higher than in other cell types. Overexpression of SRF in B cells causes the IL-2R enhancer to function as well as it does in T cells, suggesting that the high level of SRF binding in T cells is functionally important.

AB - We find that a short enhancer element containing the NF-κB binding site from the interleukin-2 receptor α-chain gene (IL-2Rα) is preferentially activated in T cells. The IL-2Rα enhancer binds NF-κB poorly and is only weakly activated by the NF-κB site alone. Serum response factor (SRF) binds to a site adjacent to the NF-κB site in the IL-2R enhancer, and both sites together have strong transcriptional activity specifically in T cells. Surprisingly, the levels of SRF constitutively expressed in T cells are consistently higher than in other cell types. Overexpression of SRF in B cells causes the IL-2R enhancer to function as well as it does in T cells, suggesting that the high level of SRF binding in T cells is functionally important.

UR - http://www.scopus.com/inward/record.url?scp=0027499605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027499605&partnerID=8YFLogxK

M3 - Article

C2 - 8455627

AN - SCOPUS:0027499605

VL - 13

SP - 2536

EP - 2545

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 4

ER -