Intensity of halothane- and hypercapnia-induced cerebral hyperemia is strain-dependent in rats

Hiroshi Takahashi, Jeffrey R. Kirsch, Toshiki Okada, Richard J. Traystman

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Cerebrovascular responses to physiologic and pharmacologic stimuli vary between laboratories using different strains of the same species. We tested whether the cerebral blood flow (CBF) response to 1% halothane or hypercapnia is strain-dependent in rats. Age-matched adult male (n = 14 of each strain) Wistar, Wistar-Kyoto (WKY), and spontaneously hypertensive rats (SHR) were anesthetized with pentobarbital and mechanically ventilated. Under baseline conditions blood flow to cerebrum (microspheres) in WKY (66 ± 5 mL · min- 1 · 100 g-1) was less than (P < 0.05) in Wistar (88 ± 5mL · min-1 · 100 g-1) and SHR (83 ± 5 mL · min-1 · 100 g-1). Blood flow to brainstem was greater (P < 0.05) in Wistar (106 ± 8 mL · min-1 · 100 g- 1) than in WKY (71 ± 5mL · min-1 · 100 g-1) and SHR (84 ± 4mL · min-1 · 100 g-1). in the halothane protocol (n = 8 each strain), administration of 1% halothane, during normocapnia, increased blood flow to the cerebrum in WKY (64 ± 6 to 120 ± 12 mL · min-1 · 100 g-1, P < 0.05) and SHR (78 ± 6 to 115 ± 8 mL · min-1 · 100 g-1, P < 0.05) but not Wistar rats (88 ± 8 to 102 ± 5 mL · min-1 · 100 g-1, not significant). Discontinuing halothane caused blood flow to return to baseline values. In the hypercapnia protocol (n = 6 each strain), exposure to 3% CO2 (to achieve a PaCO2 of 50-55 mm Hg) and 6% CO2 (to achieve a PaCO2 of 60 70 mm Hg) caused blood flow to the cerebrum to increase in Wistar (87 ± 11 to 112 ± 15 to 162 ± 23) to a similar amount as observed in WKY (69 ± 7 to 115 ± 13 to 162 ± 23 mL · min-1 · 100 g-1) but less than that observed in SHR (89 ± 7 to 174 ± 24 to 237 ± 28 mL · min-1 · 100 g- 1). These data demonstrate that the cerebral hyperemic response to vasodilator stimuli is strain-dependent in rats.

Original languageEnglish (US)
Pages (from-to)359-365
Number of pages7
JournalAnesthesia and analgesia
Volume83
Issue number2
DOIs
StatePublished - Aug 16 1996

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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