Intensity and mosaic spread analysis from PISEMA tensors in solid-state NMR

J. R. Quine, S. Achuthan, T. Asbury, R. Bertram, Michael Chapman, J. Hu, T. A. Cross

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The solid-state NMR experiment PISEMA, is a technique for determining structures of proteins, especially membrane proteins, from oriented samples. One method for determining the structure is to find orientations of local molecular frames (peptide planes) with respect to the unit magnetic field direction, B0. This is done using equations that compute the coordinates of this vector in the frames. This requires an analysis of the PISEMA function and its degeneracies. As a measure of the sensitivity of peptide plane orientations to the data, we use these equations to derive a formula for the intensity function in the powder pattern. With this function and other measures, we investigate the effect of small changes in peptide plane orientations depending on the location of the resonances in the powder pattern spectrum. This gives us an indication of the change in lineshape due to mosaic spread and a way to interpret these in terms of an orientational error bar.

Original languageEnglish (US)
Pages (from-to)190-198
Number of pages9
JournalJournal of Magnetic Resonance
Volume179
Issue number2
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

peptides
Tensors
Nuclear magnetic resonance
tensors
solid state
Powders
Peptides
nuclear magnetic resonance
proteins
Magnetic Fields
Membrane Proteins
indication
Magnetic fields
membranes
Proteins
sensitivity
magnetic fields
Experiments
Direction compound

Keywords

  • Lineshapes
  • Mosaic spread
  • PISEMA tensors
  • Powder pattern intensity
  • Solid-state NMR

ASJC Scopus subject areas

  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Intensity and mosaic spread analysis from PISEMA tensors in solid-state NMR. / Quine, J. R.; Achuthan, S.; Asbury, T.; Bertram, R.; Chapman, Michael; Hu, J.; Cross, T. A.

In: Journal of Magnetic Resonance, Vol. 179, No. 2, 04.2006, p. 190-198.

Research output: Contribution to journalArticle

Quine, JR, Achuthan, S, Asbury, T, Bertram, R, Chapman, M, Hu, J & Cross, TA 2006, 'Intensity and mosaic spread analysis from PISEMA tensors in solid-state NMR', Journal of Magnetic Resonance, vol. 179, no. 2, pp. 190-198. https://doi.org/10.1016/j.jmr.2005.12.002
Quine, J. R. ; Achuthan, S. ; Asbury, T. ; Bertram, R. ; Chapman, Michael ; Hu, J. ; Cross, T. A. / Intensity and mosaic spread analysis from PISEMA tensors in solid-state NMR. In: Journal of Magnetic Resonance. 2006 ; Vol. 179, No. 2. pp. 190-198.
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