Integrating ribosomal promoter vectors that offer a choice of constitutive expression profiles in Leishmania donovani

Radika Soysa, Khoa D. Tran, Buddy Ullman, Phillip Yates

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have designed a novel series of integrating ribosomal RNA promoter vectors with five incrementally different constitutive expression profiles, covering a 250-fold range. Differential expression was achieved by placing different combinations of synthetic or leishmanial DNA sequences upstream and downstream of the transgene coding sequence in order to modulate pre-mRNA processing efficiency and mRNA stability, respectively. All of the vectors have extensive multiple cloning sites, and versions are available for producing N- or C- terminal GFP fusions at each of the possible relative expression levels. In addition, the modular configuration of the vectors allows drug resistance cassettes and other components to be readily exchanged. In toto, these vectors should be useful additions to the toolkit available for molecular and genetic studies of Leishmania donovani.

Original languageEnglish (US)
Pages (from-to)89-92
Number of pages4
JournalMolecular and Biochemical Parasitology
Volume204
Issue number2
DOIs
StatePublished - Dec 1 2015

Fingerprint

Leishmania donovani
Ribosomal RNA
RNA Precursors
RNA Stability
Transgenes
Drug Resistance
Organism Cloning
Molecular Biology

Keywords

  • Expression vector
  • Green fluorescent protein
  • Leishmania donovani
  • Ribosomal RNA promoter

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Integrating ribosomal promoter vectors that offer a choice of constitutive expression profiles in Leishmania donovani. / Soysa, Radika; Tran, Khoa D.; Ullman, Buddy; Yates, Phillip.

In: Molecular and Biochemical Parasitology, Vol. 204, No. 2, 01.12.2015, p. 89-92.

Research output: Contribution to journalArticle

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