Insulin-like growth factor II promotes in vitro cholinergic development of mouse septal neurons: comparison with the effects of insulin-like growth factor I

Yoshihiro Konishi, Keikichi Takahashi, De Hua Chui, Ronald (Ron) Rosenfeld, Masaru Himeno, Takeshi Tabira

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We investigated the effect of insulin-like growth factors II and I (IGFII and IGFI) on septal primary cultures from mouse embryonic day 15 brains. The addition of IGFII to septal cultures enhanced total choline acetyltransferase (ChAT) activity in a dose-dependent manner. Maximal stimulation of ChAT activity was observed at 10 ng/ml IGFII. The effect of IGFII on ChAT activity was completely blocked by anti-IGFII/M-6-P receptor antibodies, whereas the antisera alone had no effect on the enzyme activity. Double-labeled immunohistochemical studies revealed that most ChAT-positive neurons expressed IGFII/M-6-P receptor immunoreactivity. These results indicate that the trophic effect of IGFII results from the direct action of this molecule through the IGFII/M-6-P receptor in septal cholinergic neurons. IGFI also stimulated ChAT activity, but with less potency than IGFII. Antibodies against the IGFII/M-6-P receptor inhibited approximately 50% of the IGFI response, suggesting that the effect of IGFI is mediated in part by the IGFII/M-6-P receptor. Thus, it appears that IGFII and IGFI are potent trophic factors for central cholinergic neurons and could potentially play a significant role in the differentiation, maintenance and regeneration of these neurons.

Original languageEnglish (US)
Pages (from-to)53-61
Number of pages9
JournalBrain Research
Volume649
Issue number1-2
DOIs
StatePublished - Jun 27 1994
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor II
Choline O-Acetyltransferase
Insulin-Like Growth Factor I
Cholinergic Agents
Neurons
Cholinergic Neurons
Antibodies
Immune Sera
Regeneration
Maintenance
In Vitro Techniques
Brain
Enzymes

Keywords

  • Cation independent mannose-6-phosphate receptor
  • Choline acetyltransferase
  • Cholinergic neuron
  • Insulin-like growth factor
  • Insulin-like growth factor II receptor
  • Neurotrophic factor

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Insulin-like growth factor II promotes in vitro cholinergic development of mouse septal neurons : comparison with the effects of insulin-like growth factor I. / Konishi, Yoshihiro; Takahashi, Keikichi; Chui, De Hua; Rosenfeld, Ronald (Ron); Himeno, Masaru; Tabira, Takeshi.

In: Brain Research, Vol. 649, No. 1-2, 27.06.1994, p. 53-61.

Research output: Contribution to journalArticle

Konishi, Yoshihiro ; Takahashi, Keikichi ; Chui, De Hua ; Rosenfeld, Ronald (Ron) ; Himeno, Masaru ; Tabira, Takeshi. / Insulin-like growth factor II promotes in vitro cholinergic development of mouse septal neurons : comparison with the effects of insulin-like growth factor I. In: Brain Research. 1994 ; Vol. 649, No. 1-2. pp. 53-61.
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