Insulin-like growth factor I (IGF-I) receptors and IGF-I action in oligodendrocytes from rat brains

Brian A. Masters, Haim Werner, Charles Roberts, Derek LeRoith, Mohan K. Raizada

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Oligodendrocyte progenitor cells were prepared by mechanical dissociation of 1-day-old rat brain cultures. These cells undergo proliferation and differentiation into oligodendrocytes as demonstrated by the expression of proliferation and differentiation-related specific antigens. We have used this unique culture system to characterize insulin-like growth factor I (IGF-I) receptors and their action in the central nervous system (CNS). 125I-IGF-I specifically binds to these cultures with high affinity. Competition-inhibition data suggest that IGF-I is most potent in competing for 125I-IGF-I binding, followed by IGF-II and insulin. Scatchard analyses of the binding data indicate a curvilinear plot with a Kd for high affinity of 0.2 nM, and a Bmax of 247 fmol/mg, and a Kd for low affinity of 3.2 nM and Bmax of 1213 fmol/mg protein. Covalent cross-linking followed by SDS-PAGE analysis demonstrated a radioactive band of Mr 135,000 which corresponds to the α subunit of the IGF-I receptor. Solution hybridization/RNase protection assay produced a single protected band corresponding to IGF-I receptor messenger RNA, further confirming the presence of these receptors. Incubation of progenitor cells with IGF-I resulted in a time- and concentration-dependent increase in [3H]thymidine incorporation and cell numbers. This effect appears to be mediated by IGF-I receptors since IGF-II and insulin were proportionately less potent. In addition to its effect on proliferation, IGF-I also increased the number of 4E7- and GC-antigen positive cells. These observations indicate that oligodendrocytes in primary culture express specific IGF-I receptors and that the interaction of IGF-I with these receptors results in the proliferation as well as differentiation of oligodendrocytes.

Original languageEnglish (US)
Pages (from-to)117-131
Number of pages15
JournalRegulatory Peptides
Volume33
Issue number2
DOIs
StatePublished - Apr 25 1991
Externally publishedYes

Fingerprint

IGF Type 1 Receptor
Oligodendroglia
Insulin-Like Growth Factor I
Rats
Brain
Insulin-Like Growth Factor II
Cell culture
Stem Cells
Insulin
Antigens
Cell proliferation
Neurology
Ribonucleases
Thymidine
Polyacrylamide Gel Electrophoresis
Cell Differentiation
Assays
Central Nervous System
Cell Count
Cells

Keywords

  • Differentiation
  • Glial progenitor
  • IGF-I receptor
  • Multiplication
  • Oligodendrocyte

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Insulin-like growth factor I (IGF-I) receptors and IGF-I action in oligodendrocytes from rat brains. / Masters, Brian A.; Werner, Haim; Roberts, Charles; LeRoith, Derek; Raizada, Mohan K.

In: Regulatory Peptides, Vol. 33, No. 2, 25.04.1991, p. 117-131.

Research output: Contribution to journalArticle

Masters, Brian A. ; Werner, Haim ; Roberts, Charles ; LeRoith, Derek ; Raizada, Mohan K. / Insulin-like growth factor I (IGF-I) receptors and IGF-I action in oligodendrocytes from rat brains. In: Regulatory Peptides. 1991 ; Vol. 33, No. 2. pp. 117-131.
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