Insulin-like growth factor binding proteins from cultured human fibroblasts. Characterization and hormonal regulation

C. A. Conover, F. Liu, D. Powell, Ronald (Ron) Rosenfeld, R. L. Hintz

Research output: Contribution to journalArticle

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Abstract

Specific, high affinity insulin-like growth factor (IGF) binding proteins are secreted by human fibroblasts in culture. By multiple criteria, the species of IGF binding proteins produced by human fibroblasts are distinct from the HepG2/amniotic fluid IGF binding protein, but share many characteristics with the growth hormone-dependent IGF binding protein forms predominant in normal adult human plasma. Treatment of cultured human fibroblasts with growth hormone produced an increase in IGF binding protein activity in the medium, while addition of glucocorticoids markedly diminished IGF binding activity. Insulin, epidermal growth factor, platelet-derived growth factor, and progesterone had no effect on IGF binding activity in fibroblast media. In comparison, HepG2 IGF binding activity was enhanced by progesterone, decreased by insulin, and unaffected by growth hormone or glucocorticoid treatment. Five molecular forms of IGF binding proteins were identified by Western ligand blots in human fibroblast conditioned medium, with M(r) = 41,500, 37,000, 32,000, 28,000, and 23,000. In human fibroblast conditioned medium, the M(r) = 41,500 and 37,000 IGF binding protein species were abundant, as in normal human plasma, with a major M(r) = 23,000 form which was a minor component in plasma.

Original languageEnglish (US)
Pages (from-to)852-859
Number of pages8
JournalJournal of Clinical Investigation
Volume83
Issue number3
StatePublished - 1989
Externally publishedYes

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Insulin-Like Growth Factor Binding Proteins
Fibroblasts
Somatomedins
Growth Hormone
Conditioned Culture Medium
Glucocorticoids
Progesterone
Insulin
Platelet-Derived Growth Factor
Amniotic Fluid
Epidermal Growth Factor
Western Blotting
Ligands
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Insulin-like growth factor binding proteins from cultured human fibroblasts. Characterization and hormonal regulation. / Conover, C. A.; Liu, F.; Powell, D.; Rosenfeld, Ronald (Ron); Hintz, R. L.

In: Journal of Clinical Investigation, Vol. 83, No. 3, 1989, p. 852-859.

Research output: Contribution to journalArticle

Conover, C. A. ; Liu, F. ; Powell, D. ; Rosenfeld, Ronald (Ron) ; Hintz, R. L. / Insulin-like growth factor binding proteins from cultured human fibroblasts. Characterization and hormonal regulation. In: Journal of Clinical Investigation. 1989 ; Vol. 83, No. 3. pp. 852-859.
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