Insulin-like growth factor binding protein-related protein 1 (IGFBP- rP1) is a potential tumor suppressor protein for prostate cancer

Cynthia C. Sprenger, Susan E. Damon, Vivian Hwa, Ron G. Rosenfeld, Stephen R. Plymate

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Insulin-like growth factor binding protein-related protein-1 (IGFBP- rP1) has been shown to have decreased expression in the progression from benign to malignant prostate epithelial cells (V. Hwa et al., J. Clin Endocrinol. Metab., 83: 4355-4362, 1998). The present study was undertaken to determine the effects of the re-expression of IGFBP-rP1 in a cell line from a model of human prostate cancer, M12, in which IGFBP-rP1 expression had been demonstrated to decrease from the parent epithelial cell, P69, to the malignant subline, M12. An IGFBP-rP1 cDNA encoding the protein was transfected into M12 cells in a plasmid that resulted in constitutive- expression of IGFBP-rP1. Clones of transfected M12 cells were selected for low (L) and high (H) levels of expression, and the plasmid vector alone was transfected into M12 as a control. After transfection, there was a marked alteration in the morphology of the M12 cells such that the H clones had an elongated appearance when compared with the M12 control cells. The M12 clones overexpressing IGFBP-rP1 had a dose-related increase in population doubling time, decreased colony formation in soft agar, an increased propensity to undergo apoptosis in response to 6-hydroxyurea, and decreased tumor formation in male athymic, nude mice. These data suggest that IGFBP-rP1 may have a suppressive effect on prostate cancer development.

Original languageEnglish (US)
Pages (from-to)2370-2375
Number of pages6
JournalCancer Research
Volume59
Issue number10
StatePublished - May 15 1999

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Sprenger, C. C., Damon, S. E., Hwa, V., Rosenfeld, R. G., & Plymate, S. R. (1999). Insulin-like growth factor binding protein-related protein 1 (IGFBP- rP1) is a potential tumor suppressor protein for prostate cancer. Cancer Research, 59(10), 2370-2375.