Insulin-like growth factor binding protein-5 gene expression is differentially regulated at a post-transcriptional level in retinoic acid-sensitive and resistant mcf-7 human breast carcinoma cells

M. Saeed Sheikh, Zhi Ming Shao, Jian Chyi Chen, David R. Clemmons, Charles Roberts, Derek LeRoith, Joseph A. Fontana

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Insulin-like growth factor binding protein (IGFBP) gene expression and IGFBP secretion were investigated in a retinoic acid (RA)-resistant subline (RROI) of MCF-7 human breast carcinoma cells. Our results demonstrate that RRO-I cells constitutively secrete higher levels of IGFBP-3 and IGFBP-5. In addition, we found that a 5-fold increase in IGFBP-5 mRNA levels observed in RRO-I cells, which eventually leads to a similar increase in the secreted levels of IGFBP-5, was partly due to an increase in the stability of IGFBP-5 mRNA. Actinomycin D and cycloheximide differentially stabilized IGFBP-5 mRNA in MCF-7 cells but not in RRO-I cells, indicating a difference in the control of IGFBP-5 gene regulation at the level of mRNA stability in these cell lines.

Original languageEnglish (US)
Pages (from-to)1122-1130
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume188
Issue number3
DOIs
StatePublished - Nov 16 1992
Externally publishedYes

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Insulin-Like Growth Factor Binding Protein 5
Tretinoin
Gene expression
Cells
Breast Neoplasms
Gene Expression
Insulin-Like Growth Factor Binding Proteins
Messenger RNA
Insulin-Like Growth Factor Binding Protein 3
MCF-7 Cells
RNA Stability
Dactinomycin
Cycloheximide
Cell Line
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Insulin-like growth factor binding protein-5 gene expression is differentially regulated at a post-transcriptional level in retinoic acid-sensitive and resistant mcf-7 human breast carcinoma cells. / Sheikh, M. Saeed; Shao, Zhi Ming; Chen, Jian Chyi; Clemmons, David R.; Roberts, Charles; LeRoith, Derek; Fontana, Joseph A.

In: Biochemical and Biophysical Research Communications, Vol. 188, No. 3, 16.11.1992, p. 1122-1130.

Research output: Contribution to journalArticle

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abstract = "Insulin-like growth factor binding protein (IGFBP) gene expression and IGFBP secretion were investigated in a retinoic acid (RA)-resistant subline (RROI) of MCF-7 human breast carcinoma cells. Our results demonstrate that RRO-I cells constitutively secrete higher levels of IGFBP-3 and IGFBP-5. In addition, we found that a 5-fold increase in IGFBP-5 mRNA levels observed in RRO-I cells, which eventually leads to a similar increase in the secreted levels of IGFBP-5, was partly due to an increase in the stability of IGFBP-5 mRNA. Actinomycin D and cycloheximide differentially stabilized IGFBP-5 mRNA in MCF-7 cells but not in RRO-I cells, indicating a difference in the control of IGFBP-5 gene regulation at the level of mRNA stability in these cell lines.",
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AU - Fontana, Joseph A.

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