Insulin-like growth factor binding protein-1 in the ruminant uterus: potential endometrial marker and regulator of conceptus elongation

Rebecca M. Simmons, David Erikson, Jinyoung Kim, Robert C. Burghardt, Fuller W. Bazer, Greg A. Johnson, Thomas E. Spencer

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Establishment of pregnancy in ruminants requires conceptus elongation and production of interferon-τ (IFNT), the pregnancy recognition signal that maintains ovarian progesterone (P4) production. These studies determined temporal and spatial alterations in IGF binding protein (IGFBP)-1 and IGFBP3 in the ovine and bovine uterus; effects of P4 and IFNT on their expression in the ovine uterus; and effects of IGFBP1 on ovine trophectoderm cell proliferation, migration, and attachment. IGFBP1 and IGFBP3 were studied because they are the only IGFBPs specifically expressed by the endometrial luminal epithelia in sheep. In sheep, IGFBP1 and IGFBP3 expression was coordinate with the period of conceptus elongation, whereas only IGFBP1 expression was coordinate with conceptus elongation in cattle. IGFBP1 mRNA in the ovine endometria was between 5- and 29-fold more abundant between d 12 and 16 of pregnancy compared with the estrous cycle and greater on d 16 of pregnancy than nonpregnancy in the bovine uterus. In sheep, P4 induced and IFNT stimulated expression of IGFBP1 but not IGFBP3; however, the effect of IFNT did not mimic the abundant increase observed in pregnant ewes. Therefore, IGFBP1 expression in the endometrium is regulated by another factor from the conceptus. IGFBP1 did not affect the proliferation of ovine trophectoderm cells in vitro but did stimulate their migration and mediate their attachment. These studies reveal that IGFBP1 is a common endometrial marker of conceptus elongation in sheep and cattle and most likely regulates conceptus elongation by stimulating migration and attachment of the trophectoderm.

Original languageEnglish (US)
Pages (from-to)4295-4305
Number of pages11
JournalEndocrinology
Volume150
Issue number9
DOIs
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor Binding Protein 1
Ruminants
Uterus
Sheep
Pregnancy
Endometrium
Insulin-Like Growth Factor Binding Proteins
Estrous Cycle
Interferons
Cell Movement
Progesterone
Epithelium
Cell Proliferation
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology

Cite this

Simmons, R. M., Erikson, D., Kim, J., Burghardt, R. C., Bazer, F. W., Johnson, G. A., & Spencer, T. E. (2009). Insulin-like growth factor binding protein-1 in the ruminant uterus: potential endometrial marker and regulator of conceptus elongation. Endocrinology, 150(9), 4295-4305. https://doi.org/10.1210/en.2009-0060

Insulin-like growth factor binding protein-1 in the ruminant uterus : potential endometrial marker and regulator of conceptus elongation. / Simmons, Rebecca M.; Erikson, David; Kim, Jinyoung; Burghardt, Robert C.; Bazer, Fuller W.; Johnson, Greg A.; Spencer, Thomas E.

In: Endocrinology, Vol. 150, No. 9, 09.2009, p. 4295-4305.

Research output: Contribution to journalArticle

Simmons, RM, Erikson, D, Kim, J, Burghardt, RC, Bazer, FW, Johnson, GA & Spencer, TE 2009, 'Insulin-like growth factor binding protein-1 in the ruminant uterus: potential endometrial marker and regulator of conceptus elongation', Endocrinology, vol. 150, no. 9, pp. 4295-4305. https://doi.org/10.1210/en.2009-0060
Simmons, Rebecca M. ; Erikson, David ; Kim, Jinyoung ; Burghardt, Robert C. ; Bazer, Fuller W. ; Johnson, Greg A. ; Spencer, Thomas E. / Insulin-like growth factor binding protein-1 in the ruminant uterus : potential endometrial marker and regulator of conceptus elongation. In: Endocrinology. 2009 ; Vol. 150, No. 9. pp. 4295-4305.
@article{d1ecc5864174445ab197dd6d50fd51ff,
title = "Insulin-like growth factor binding protein-1 in the ruminant uterus: potential endometrial marker and regulator of conceptus elongation",
abstract = "Establishment of pregnancy in ruminants requires conceptus elongation and production of interferon-τ (IFNT), the pregnancy recognition signal that maintains ovarian progesterone (P4) production. These studies determined temporal and spatial alterations in IGF binding protein (IGFBP)-1 and IGFBP3 in the ovine and bovine uterus; effects of P4 and IFNT on their expression in the ovine uterus; and effects of IGFBP1 on ovine trophectoderm cell proliferation, migration, and attachment. IGFBP1 and IGFBP3 were studied because they are the only IGFBPs specifically expressed by the endometrial luminal epithelia in sheep. In sheep, IGFBP1 and IGFBP3 expression was coordinate with the period of conceptus elongation, whereas only IGFBP1 expression was coordinate with conceptus elongation in cattle. IGFBP1 mRNA in the ovine endometria was between 5- and 29-fold more abundant between d 12 and 16 of pregnancy compared with the estrous cycle and greater on d 16 of pregnancy than nonpregnancy in the bovine uterus. In sheep, P4 induced and IFNT stimulated expression of IGFBP1 but not IGFBP3; however, the effect of IFNT did not mimic the abundant increase observed in pregnant ewes. Therefore, IGFBP1 expression in the endometrium is regulated by another factor from the conceptus. IGFBP1 did not affect the proliferation of ovine trophectoderm cells in vitro but did stimulate their migration and mediate their attachment. These studies reveal that IGFBP1 is a common endometrial marker of conceptus elongation in sheep and cattle and most likely regulates conceptus elongation by stimulating migration and attachment of the trophectoderm.",
author = "Simmons, {Rebecca M.} and David Erikson and Jinyoung Kim and Burghardt, {Robert C.} and Bazer, {Fuller W.} and Johnson, {Greg A.} and Spencer, {Thomas E.}",
year = "2009",
month = "9",
doi = "10.1210/en.2009-0060",
language = "English (US)",
volume = "150",
pages = "4295--4305",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "9",

}

TY - JOUR

T1 - Insulin-like growth factor binding protein-1 in the ruminant uterus

T2 - potential endometrial marker and regulator of conceptus elongation

AU - Simmons, Rebecca M.

AU - Erikson, David

AU - Kim, Jinyoung

AU - Burghardt, Robert C.

AU - Bazer, Fuller W.

AU - Johnson, Greg A.

AU - Spencer, Thomas E.

PY - 2009/9

Y1 - 2009/9

N2 - Establishment of pregnancy in ruminants requires conceptus elongation and production of interferon-τ (IFNT), the pregnancy recognition signal that maintains ovarian progesterone (P4) production. These studies determined temporal and spatial alterations in IGF binding protein (IGFBP)-1 and IGFBP3 in the ovine and bovine uterus; effects of P4 and IFNT on their expression in the ovine uterus; and effects of IGFBP1 on ovine trophectoderm cell proliferation, migration, and attachment. IGFBP1 and IGFBP3 were studied because they are the only IGFBPs specifically expressed by the endometrial luminal epithelia in sheep. In sheep, IGFBP1 and IGFBP3 expression was coordinate with the period of conceptus elongation, whereas only IGFBP1 expression was coordinate with conceptus elongation in cattle. IGFBP1 mRNA in the ovine endometria was between 5- and 29-fold more abundant between d 12 and 16 of pregnancy compared with the estrous cycle and greater on d 16 of pregnancy than nonpregnancy in the bovine uterus. In sheep, P4 induced and IFNT stimulated expression of IGFBP1 but not IGFBP3; however, the effect of IFNT did not mimic the abundant increase observed in pregnant ewes. Therefore, IGFBP1 expression in the endometrium is regulated by another factor from the conceptus. IGFBP1 did not affect the proliferation of ovine trophectoderm cells in vitro but did stimulate their migration and mediate their attachment. These studies reveal that IGFBP1 is a common endometrial marker of conceptus elongation in sheep and cattle and most likely regulates conceptus elongation by stimulating migration and attachment of the trophectoderm.

AB - Establishment of pregnancy in ruminants requires conceptus elongation and production of interferon-τ (IFNT), the pregnancy recognition signal that maintains ovarian progesterone (P4) production. These studies determined temporal and spatial alterations in IGF binding protein (IGFBP)-1 and IGFBP3 in the ovine and bovine uterus; effects of P4 and IFNT on their expression in the ovine uterus; and effects of IGFBP1 on ovine trophectoderm cell proliferation, migration, and attachment. IGFBP1 and IGFBP3 were studied because they are the only IGFBPs specifically expressed by the endometrial luminal epithelia in sheep. In sheep, IGFBP1 and IGFBP3 expression was coordinate with the period of conceptus elongation, whereas only IGFBP1 expression was coordinate with conceptus elongation in cattle. IGFBP1 mRNA in the ovine endometria was between 5- and 29-fold more abundant between d 12 and 16 of pregnancy compared with the estrous cycle and greater on d 16 of pregnancy than nonpregnancy in the bovine uterus. In sheep, P4 induced and IFNT stimulated expression of IGFBP1 but not IGFBP3; however, the effect of IFNT did not mimic the abundant increase observed in pregnant ewes. Therefore, IGFBP1 expression in the endometrium is regulated by another factor from the conceptus. IGFBP1 did not affect the proliferation of ovine trophectoderm cells in vitro but did stimulate their migration and mediate their attachment. These studies reveal that IGFBP1 is a common endometrial marker of conceptus elongation in sheep and cattle and most likely regulates conceptus elongation by stimulating migration and attachment of the trophectoderm.

UR - http://www.scopus.com/inward/record.url?scp=69249138378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69249138378&partnerID=8YFLogxK

U2 - 10.1210/en.2009-0060

DO - 10.1210/en.2009-0060

M3 - Article

C2 - 19497977

AN - SCOPUS:69249138378

VL - 150

SP - 4295

EP - 4305

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 9

ER -