Insights into transport mechanism from LeuT engineered to transport tryptophan

Chayne L. Piscitelli, Eric Gouaux

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

LeuT is a bacterial homologue of the neurotransmitter:sodium symporter (NSS) family and, being the only NSS member to have been structurally characterized by X-ray crystallography, is a model protein for studying transporter structure and mechanism. Transport activity in LeuT was hypothesized to require structural transitions between open-to-out and occluded conformations dependent upon protein:ligand binding complementarity. Here, using crystallographic and functional analysis, we show that binding site modification produces changes in both structure and activity that are consistent with complementarity-dependent structural transitions to the occluded state. The mutation I359Q converts the activity of tryptophan from inhibitor to transportable substrate. This mutation changes the local environment of the binding site, inducing the bound tryptophan to adopt a different conformer than in the wild-type complex. Instead of trapping the transporter open, tryptophan binding now allows the formation of an occluded state. Thus, transport activity is correlated to the ability of the ligand to promote the structural transition to the occluded state, a step in the transport cycle that is dependent on protein:ligand complementarity in the central binding site.

Original languageEnglish (US)
Pages (from-to)228-235
Number of pages8
JournalEMBO Journal
Volume31
Issue number1
DOIs
StatePublished - Jan 4 2012

Fingerprint

Plasma Membrane Neurotransmitter Transport Proteins
Tryptophan
Binding Sites
Ligands
Functional analysis
Mutation
Proteins
X ray crystallography
X Ray Crystallography
Protein Binding
Conformations
Substrates

Keywords

  • crystallography
  • LeuT
  • mechanism
  • transporter
  • tryptophan

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Insights into transport mechanism from LeuT engineered to transport tryptophan. / Piscitelli, Chayne L.; Gouaux, Eric.

In: EMBO Journal, Vol. 31, No. 1, 04.01.2012, p. 228-235.

Research output: Contribution to journalArticle

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