@inbook{c7f7157463be4559a9bb0e292fedbcb7,
title = "Insights from Bacterial Subtilases into the Mechanisms of Intramolecular Chaperone-Mediated Activation of Furin",
abstract = "Prokaryotic subtilisins and eukaryotic proprotein convertases (PCs) are two homologous protease subfamilies that belong to the larger ubiquitous super-family called subtilases. Members of the subtilase super-family are produced as zymogens wherein their propeptide domains function as dedicated intramolecular chaperones (IMCs) that facilitate correct folding and regulate precise activation of their cognate catalytic domains. The molecular and cellular determinants that modulate IMC-dependent folding and activation of PCs are poorly understood. In this chapter we review what we have learned from the folding and activation of prokaryotic subtilisin, discuss how this has molded our understanding of furin maturation, and foray into the concept of pH sensors, which may represent a paradigm that PCs (and possibly other IMC-dependent eukaryotic proteins) follow for regulating their biological functions using the pH gradient in the secretory pathway.",
keywords = "Intramolecular chaperones, histidine protonation, pH sensors, proprotein convertases, protease activation and regulation, secretory pathway, subtilases",
author = "Ujwal Shinde and Gary Thomas",
note = "Funding Information: Our apologies to colleagues whose work we did not cite because of space limitations. Special thanks go to Jimmy Dikeakos, David Radler, Laura Figoski, and Stephanie Dillon for insightful discussions, for review of the manuscript, and for editing. U.S. is supported by grants from the National Science Foundation (NSF-0746589) and the Oregon Nanoscience and Microtech-nologies Institute and G.T. is supported by grant from the National Institutes of Health (DK37274 and CA151564). Publisher Copyright: {\textcopyright} 2011, Springer Science+Business Media, LLC.",
year = "2011",
doi = "10.1007/978-1-61779-204-5_4",
language = "English (US)",
isbn = "9781617792038",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "59--106",
booktitle = "Proprotein Convertases",
}