Inner ear tissue remodeling and ion homeostasis gene alteration in murine chronic otitis media

Carol Macarthur, Fran Hausman, J. Beth Kempton, Nathan Sautter, Dennis Trune

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

HYPOTHESIS: Studies were designed to ascertain the impact of chronic middle ear infection on the numerous ion and water channels, transporters, and tissue remodeling genes in the inner and middle ear. BACKGROUND: Permanent sensorineural hearing loss is a significant problem resulting from chronic middle ear disease, although the inner ear processes involved are poorly defined. Maintaining a balanced ionic composition of endolymph in the inner ear is crucial for hearing; thus, it was hypothesized that this may be at risk with inflammation. METHODS: Inner and middle ear RNA collected separately from 6-month-old C3H/HeJ mice with prolonged middle ear disease were subjected to qRT-PCR for 8 common inflammatory cytokine genes, 24 genes for channels controlling ion (sodium, potassium, and chloride) and water (aquaporin) transport, tight junction claudins, and gap junction connexins, and 32 tissue remodeling genes. Uninfected Balb/c mice were used as controls. RESULTS: Significant increase in inner ear inflammatory and ion homeostasis (claudin, aquaporin, and gap junction) gene expression, and both upregulation and downregulation of tissue remodeling gene expression occurred. Alteration in middle ear ion homeostasis and tissue remodeling gene expression was noted in the setting of uniform upregulation of cytokine genes. CONCLUSION: Chronic inflammatory middle ear disease can impact inner ear ion and water transport functions and induce tissue remodeling. Recognizing these inner ear mechanisms at risk may identify potential therapeutic targets to maintain hearing during prolonged otitis media.

Original languageEnglish (US)
Pages (from-to)338-346
Number of pages9
JournalOtology and Neurotology
Volume34
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Otitis Media
Middle Ear
Inner Ear
Homeostasis
Ions
Ear Diseases
Aquaporins
Genes
Gap Junctions
Ion Channels
Gene Expression
Hearing
Up-Regulation
Claudins
Endolymph
Cytokines
Potassium Chloride
Water
Inbred C3H Mouse
Sensorineural Hearing Loss

Keywords

  • Chronic otitis media
  • Geneexpression
  • Hearing loss
  • Ion homeostasis
  • Mouse model
  • Tissue remodeling

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Clinical Neurology
  • Sensory Systems
  • Medicine(all)

Cite this

Inner ear tissue remodeling and ion homeostasis gene alteration in murine chronic otitis media. / Macarthur, Carol; Hausman, Fran; Kempton, J. Beth; Sautter, Nathan; Trune, Dennis.

In: Otology and Neurotology, Vol. 34, No. 2, 02.2013, p. 338-346.

Research output: Contribution to journalArticle

Macarthur, Carol ; Hausman, Fran ; Kempton, J. Beth ; Sautter, Nathan ; Trune, Dennis. / Inner ear tissue remodeling and ion homeostasis gene alteration in murine chronic otitis media. In: Otology and Neurotology. 2013 ; Vol. 34, No. 2. pp. 338-346.
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