Innate immunity in Caenorhabditis elegans is regulated by neurons expressing NPR-1/GPCR

Katie L. Styer, Varsha Singh, Evan Macosko, Sarah E. Steele, Cornelia I. Bargmann, Alejandro Aballay

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

A large body of evidence indicates that metazoan innate immunity is regulated by the nervous system, but the mechanisms involved in the process and the biological importance of such control remain unclear. We show that a neural circuit involving npr-1, which encodes a G protein-coupled receptor (GPCR) related to mammalian neuropeptide Y receptors, functions to suppress innate immune responses. The immune inhibitory function requires a guanosine 3′,5′-monophosphate-gated ion channel encoded by tax-2 and tax-4 as well as the soluble guanylate cyclase GCY-35. Furthermore, we show that npr-1- and gcy-35-expressing sensory neurons actively suppress immune responses of nonneuronal tissues. A full-genome microarray analysis on animals with altered neural function due to mutation in npr-1 shows an enrichment in genes that are markers of innate immune responses, including those regulated by a conserved PMK-1/p38 mitogen-activated protein kinase signaling pathway. These results present evidence that neurons directly control innate immunity in C. elegans, suggesting that GPCRs may participate in neural circuits that receive inputs from either pathogens or infected sites and integrate them to coordinate appropriate immune responses.

Original languageEnglish (US)
Pages (from-to)460-464
Number of pages5
JournalScience
Volume322
Issue number5900
DOIs
StatePublished - Oct 17 2008
Externally publishedYes

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Caenorhabditis elegans
G-Protein-Coupled Receptors
Innate Immunity
Neurons
Neuropeptide Y Receptors
Guanosine Monophosphate
Biological Phenomena
p38 Mitogen-Activated Protein Kinases
Sensory Receptor Cells
Microarray Analysis
Ion Channels
Nervous System
Genome
Mutation
Genes

ASJC Scopus subject areas

  • General

Cite this

Styer, K. L., Singh, V., Macosko, E., Steele, S. E., Bargmann, C. I., & Aballay, A. (2008). Innate immunity in Caenorhabditis elegans is regulated by neurons expressing NPR-1/GPCR. Science, 322(5900), 460-464. https://doi.org/10.1126/science.1163673

Innate immunity in Caenorhabditis elegans is regulated by neurons expressing NPR-1/GPCR. / Styer, Katie L.; Singh, Varsha; Macosko, Evan; Steele, Sarah E.; Bargmann, Cornelia I.; Aballay, Alejandro.

In: Science, Vol. 322, No. 5900, 17.10.2008, p. 460-464.

Research output: Contribution to journalArticle

Styer, KL, Singh, V, Macosko, E, Steele, SE, Bargmann, CI & Aballay, A 2008, 'Innate immunity in Caenorhabditis elegans is regulated by neurons expressing NPR-1/GPCR', Science, vol. 322, no. 5900, pp. 460-464. https://doi.org/10.1126/science.1163673
Styer, Katie L. ; Singh, Varsha ; Macosko, Evan ; Steele, Sarah E. ; Bargmann, Cornelia I. ; Aballay, Alejandro. / Innate immunity in Caenorhabditis elegans is regulated by neurons expressing NPR-1/GPCR. In: Science. 2008 ; Vol. 322, No. 5900. pp. 460-464.
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