Initiation of periovulatory events in gonadotrophin-stimulated macaques with varying doses of recombinant human chorionic gonadotrophin

M. B. Zelinski-Wooten, J. S. Hutchison, I. Trinchard-Lugan, D. L. Hess, D. P. Wolf, R. L. Stouffer

    Research output: Contribution to journalArticle

    49 Scopus citations

    Abstract

    During in-vitro fertilization (IVF) cycles, a large bolus of human chorionic gonadotrophin (HCG) is used to induce periovulatory events, but the efficacy of lower doses is undefined. Following follicular stimulation in rhesus monkeys, oocyte nuclear maturation, IVF, granulosa cell luteinization and corpus luteum function were compared after injection of 100, 300 or 1000 IU recombinant HCG or 1000 IU urinary HCG. Bioactive HCG rose to peak concentrations within 2 h that were proportional to the dose administered (100 < 300 < 1000 IU, recombinant HCG = urinary HCG). The duration of surge values ( > 100 ng/ml) was also dose-dependent (0 h, 100 IU; 24 h, 300 IU; > 48 h, 1000 IU, recombinant and urinary HCG). While the proportions of oocytes resuming meiosis and undergoing IVF were similar among groups, fewer animals yielded fertilizable oocytes following 100 and 300 IU (five of nine) compared to 1000 IU recombinant and urinary HCG (nine of 10). Peak values of serum progesterone in the luteal phase were similar, but declined 2 days earlier after 100 and 300 IU relative to 1000 IU recombinant and urinary HCG. Thus, 3-10 fold lower doses of HCG elicit low amplitude surges of short duration that induce periovulatory events such as re-initiation of oocyte meiosis and granulosa cell luteinization. However, oocyte fertilization and luteal function may optimally require surges of higher amplitude and longer duration similar to those produced by standard doses of 1000 IU recombinant or urinary HCG.

    Original languageEnglish (US)
    Pages (from-to)1877-1885
    Number of pages9
    JournalHuman Reproduction
    Volume12
    Issue number9
    DOIs
    StatePublished - Jan 1 1997

    Keywords

    • Gonadotrophin surge
    • Granulosa cell
    • In-vitro fertilization
    • Oocyte maturation
    • Recombinant HCG

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Obstetrics and Gynecology

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