Inhibitory effects of incomplete Freund's adjuvant on experimental autoimmune encephalomyelitis

Alex Zamora; Agata Matejuk; Marc Silverman; Arthur A. Vandenbark; Halina Offner

doi:10.1080/08916930290005873

Inhibitory effects of incomplete Freund's adjuvant on experimental autoimmune encephalomyelitis

Alex Zamora, Agata Matejuk, Marc Silverman, Arthur A. Vandenbark, Halina Offner

Neurology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Freund's incomplete adjuvant (IFA), an aqueous/oil emulsion that is widely used in combination with antigenic proteins and peptides to induce tolerance, is considered to be immunologically inert. However, sporadic reports indicate that IFA may itself have inhibitory properties on induction of adjuvant induced arthritis and spontaneous diabetes. In the current study, the effects of IFA/saline were evaluated on the induction of experimental autoimmune encephalomyelitis (EAE) in three different strains of mice. IFA/saline given i.p. in two doses of >100 μl 10 days apart were found to inhibit EAE induction to varying degrees in all three strains of mice in a dose dependent fashion. The IFA/saline injections inhibited both mitogen and antigen-induced T cell proliferation, induced elevated secretion of IFN-γ and IL-10 by neuroantigen specific T cells, and reduced expression of cytokines, chemokines, and chemokine receptors of CNS-infiltrating mononuclear cells. These data demonstrate for the first time a direct inhibitory effect of IFA/saline on EAE, and re-emphasize the need to properly control experiments using IFA to induce antigen-specific tolerance.

Original language	English (US)
Pages (from-to)	21-28
Number of pages	8
Journal	Autoimmunity
Volume	35
Issue number	1
DOIs	https://doi.org/10.1080/08916930290005873
State	Published - 2002

Keywords

EAE
IFA
IL10
MBP Ac1-11
γINF

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1080/08916930290005873

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title = "Inhibitory effects of incomplete Freund's adjuvant on experimental autoimmune encephalomyelitis",

abstract = "Freund's incomplete adjuvant (IFA), an aqueous/oil emulsion that is widely used in combination with antigenic proteins and peptides to induce tolerance, is considered to be immunologically inert. However, sporadic reports indicate that IFA may itself have inhibitory properties on induction of adjuvant induced arthritis and spontaneous diabetes. In the current study, the effects of IFA/saline were evaluated on the induction of experimental autoimmune encephalomyelitis (EAE) in three different strains of mice. IFA/saline given i.p. in two doses of >100 μl 10 days apart were found to inhibit EAE induction to varying degrees in all three strains of mice in a dose dependent fashion. The IFA/saline injections inhibited both mitogen and antigen-induced T cell proliferation, induced elevated secretion of IFN-γ and IL-10 by neuroantigen specific T cells, and reduced expression of cytokines, chemokines, and chemokine receptors of CNS-infiltrating mononuclear cells. These data demonstrate for the first time a direct inhibitory effect of IFA/saline on EAE, and re-emphasize the need to properly control experiments using IFA to induce antigen-specific tolerance.",

keywords = "EAE, IFA, IL10, MBP Ac1-11, γINF",

author = "Alex Zamora and Agata Matejuk and Marc Silverman and Vandenbark, {Arthur A.} and Halina Offner",

note = "Funding Information: The authors wish to thank Ms Eva Niehaus for assistance in preparing the manuscript. This work was supported by Grants AI42376, NS23221 and NS23444 from the National Institutes of Health, the National Multiple Sclerosis Society, the Nancy Davis Center Without Walls, and the US Department of Veterans Affairs.",

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AU - Silverman, Marc

AU - Vandenbark, Arthur A.

AU - Offner, Halina

N1 - Funding Information: The authors wish to thank Ms Eva Niehaus for assistance in preparing the manuscript. This work was supported by Grants AI42376, NS23221 and NS23444 from the National Institutes of Health, the National Multiple Sclerosis Society, the Nancy Davis Center Without Walls, and the US Department of Veterans Affairs.

PY - 2002

Y1 - 2002

N2 - Freund's incomplete adjuvant (IFA), an aqueous/oil emulsion that is widely used in combination with antigenic proteins and peptides to induce tolerance, is considered to be immunologically inert. However, sporadic reports indicate that IFA may itself have inhibitory properties on induction of adjuvant induced arthritis and spontaneous diabetes. In the current study, the effects of IFA/saline were evaluated on the induction of experimental autoimmune encephalomyelitis (EAE) in three different strains of mice. IFA/saline given i.p. in two doses of >100 μl 10 days apart were found to inhibit EAE induction to varying degrees in all three strains of mice in a dose dependent fashion. The IFA/saline injections inhibited both mitogen and antigen-induced T cell proliferation, induced elevated secretion of IFN-γ and IL-10 by neuroantigen specific T cells, and reduced expression of cytokines, chemokines, and chemokine receptors of CNS-infiltrating mononuclear cells. These data demonstrate for the first time a direct inhibitory effect of IFA/saline on EAE, and re-emphasize the need to properly control experiments using IFA to induce antigen-specific tolerance.

AB - Freund's incomplete adjuvant (IFA), an aqueous/oil emulsion that is widely used in combination with antigenic proteins and peptides to induce tolerance, is considered to be immunologically inert. However, sporadic reports indicate that IFA may itself have inhibitory properties on induction of adjuvant induced arthritis and spontaneous diabetes. In the current study, the effects of IFA/saline were evaluated on the induction of experimental autoimmune encephalomyelitis (EAE) in three different strains of mice. IFA/saline given i.p. in two doses of >100 μl 10 days apart were found to inhibit EAE induction to varying degrees in all three strains of mice in a dose dependent fashion. The IFA/saline injections inhibited both mitogen and antigen-induced T cell proliferation, induced elevated secretion of IFN-γ and IL-10 by neuroantigen specific T cells, and reduced expression of cytokines, chemokines, and chemokine receptors of CNS-infiltrating mononuclear cells. These data demonstrate for the first time a direct inhibitory effect of IFA/saline on EAE, and re-emphasize the need to properly control experiments using IFA to induce antigen-specific tolerance.

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Inhibitory effects of incomplete Freund's adjuvant on experimental autoimmune encephalomyelitis

Abstract

Keywords

ASJC Scopus subject areas

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