Inhibition of salivary secretion by lipopolysaccharide: Possible role of prostaglandins

A. Lomniczi, C. Mohn, A. Faletti, A. Franchi, S. M. McCann, V. Rettori, J. C. Elverdin

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    Abstract

    Inducible (calcium-independent) nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) are important in the regulation of the function of different organs during infection. A single dose of lipopolysaccharide (LPS; 5 mg/kg ip) within 6 h increased NOS activity (20%) and prostaglandin E (PGE) content (100%) in submandibular glands (SMG) and blocked stimulated salivary secretion in adult male rats. The administration of an iNOS synthesis inhibitor, aminoguanidine (AG), with LPS decreased NOS activity and PGE content. Furthermore, the administration of meloxicam (MLX), an inhibitor of COX-2, blocked the increase in PGE and the production of NO. The incubation of slices of SMG in the presence of 3-morpholinosydnonimine, a donor of NO, increased the release of PGE highly significantly. The incubation of SMG in the presence of a PGE 1 analog (alprostadil) increased the production of NO. These results indicate that LPS activates NOS, leading to NO release, which activates COX, generating PGEs that act back to further activate NOS, causing further generation of PGEs by activation of COX. Because the alprostadil administration inhibited stimulated salivation, LPS-induced inhibition of salivation appears to be caused by increased PGE production. Diminished salivary secretion produces poor oral health; thus the use of COX-2 inhibitors to counteract the effects of inhibited salivation should be considered.

    Original languageEnglish (US)
    Pages (from-to)E405-E411
    JournalAmerican Journal of Physiology - Endocrinology and Metabolism
    Volume281
    Issue number2 44-2
    StatePublished - Aug 27 2001

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    Keywords

    • Cyclooxygenase
    • Endotoxemia
    • Methacholine
    • Nitric oxide synthase
    • Noradrenaline

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism
    • Physiology
    • Physiology (medical)

    Cite this

    Lomniczi, A., Mohn, C., Faletti, A., Franchi, A., McCann, S. M., Rettori, V., & Elverdin, J. C. (2001). Inhibition of salivary secretion by lipopolysaccharide: Possible role of prostaglandins. American Journal of Physiology - Endocrinology and Metabolism, 281(2 44-2), E405-E411.