Inhibition of protein-tyrosine kinase activity in intact cells by the aptameric action of oligodeoxynucleotides

Raymond Bergan, E. Kyle, Y. Connell, L. Neckers

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Abstract

Direct interaction of oligodeoxynucleotides (ODNs) with proteins represents one of the nonantisense-mediated effects of ODNs. Phosphorothioate-capped ODNs have been shown to inhibit directly the in vitro kinase activity of the chronic myelogenous leukemia-associated protein-tyrosine kinase p210(bcr-abl). In this study we have determined the efficacy of this aptameric ODN in a cellular system using the K562 chronic myelogenous leukemia-derived cell line. Significant effects upon cellular phosphotyrosine content, as well as cellular growth in soft agar, are observed. These effects are sequence specific and are not mediated through changes in p210(bcr-abl) protein levels. Additional ODNs are described that also reduce cellular phosphotyrosine levels and inhibit growth in soft agar but do not inhibit p210(bcr-abl) kinase activity in vitro.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalAntisense Research and Development
Volume5
Issue number1
Publication statusPublished - 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

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