Inhibition of morphogenetic movement during Xenopus gastrulation by injected sulfatase: Implications for anteroposterior and dorsoventral axis formation

John B. Wallingford, Amy K. Sater, J. Akif Uzman, Michael V. Danilchik

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

In order to explore the role of morphogenetic movement in the establishment of anteroposterior and dorsoventral axes, we sought to identify novel in vivo inhibitors of gastrulation movements in Xenopus laevis. Injection of hydrolytic sulfatase into the blastocoels of gastrula stage embryos resulted in severe anteroposterior truncation, without a corresponding truncation of the dorsoventral axis. Confocal microscopy of whole embryos revealed that gastrulation movements are severely disrupted by sulfatase; in addition, sulfatase dramatically inhibited chordomesodermal cell elongation and convergent extension movements in planar dorsal marginal zone explants. The phenotype of anteroposterior reduction elicited by sulfatase is distinctly different from commonly generated dorsoanterior phenotypes (e.g., ultraviolet irradiation of the vegetal cortex prior to cortical rotation or suramin injection), and the two varieties of phenotype appear to result from inhibition of distinct, separable components of the axis-generating machinery.

Original languageEnglish (US)
Pages (from-to)224-235
Number of pages12
JournalDevelopmental Biology
Volume187
Issue number2
DOIs
StatePublished - Jul 15 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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