TY - JOUR
T1 - Inhibition of morphogenetic movement during Xenopus gastrulation by injected sulfatase
T2 - Implications for anteroposterior and dorsoventral axis formation
AU - Wallingford, John B.
AU - Sater, Amy K.
AU - Uzman, J. Akif
AU - Danilchik, Michael V.
N1 - Funding Information:
The authors thank B. Brown, J. Christian, K. Larkin, and P. Vize for their helpful comments. J.B.W. would like to thank Alice Kagi, an inspiring teacher. The authors also acknowledge Dick Dale for his enormous contribution. M.V.D. was supported by NSF DCB-8916614, March of Dimes BOC 5-721, and a project grant from Wesleyan University to M.V.D. J.B.W. was supported by an Undergraduate Research Fellowship from Wesleyan University's Hughes Program in the Life Sciences and at UT Austin by Texas Advanced Research Program 187 to Peter Vize. Work in the laboratory of A.K.S. was supported by NSF DCB-9118746 and by Texas Advanced Research Program 131.
PY - 1997/7/15
Y1 - 1997/7/15
N2 - In order to explore the role of morphogenetic movement in the establishment of anteroposterior and dorsoventral axes, we sought to identify novel in vivo inhibitors of gastrulation movements in Xenopus laevis. Injection of hydrolytic sulfatase into the blastocoels of gastrula stage embryos resulted in severe anteroposterior truncation, without a corresponding truncation of the dorsoventral axis. Confocal microscopy of whole embryos revealed that gastrulation movements are severely disrupted by sulfatase; in addition, sulfatase dramatically inhibited chordomesodermal cell elongation and convergent extension movements in planar dorsal marginal zone explants. The phenotype of anteroposterior reduction elicited by sulfatase is distinctly different from commonly generated dorsoanterior phenotypes (e.g., ultraviolet irradiation of the vegetal cortex prior to cortical rotation or suramin injection), and the two varieties of phenotype appear to result from inhibition of distinct, separable components of the axis-generating machinery.
AB - In order to explore the role of morphogenetic movement in the establishment of anteroposterior and dorsoventral axes, we sought to identify novel in vivo inhibitors of gastrulation movements in Xenopus laevis. Injection of hydrolytic sulfatase into the blastocoels of gastrula stage embryos resulted in severe anteroposterior truncation, without a corresponding truncation of the dorsoventral axis. Confocal microscopy of whole embryos revealed that gastrulation movements are severely disrupted by sulfatase; in addition, sulfatase dramatically inhibited chordomesodermal cell elongation and convergent extension movements in planar dorsal marginal zone explants. The phenotype of anteroposterior reduction elicited by sulfatase is distinctly different from commonly generated dorsoanterior phenotypes (e.g., ultraviolet irradiation of the vegetal cortex prior to cortical rotation or suramin injection), and the two varieties of phenotype appear to result from inhibition of distinct, separable components of the axis-generating machinery.
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U2 - 10.1006/dbio.1997.8571
DO - 10.1006/dbio.1997.8571
M3 - Article
C2 - 9242419
AN - SCOPUS:0030798034
SN - 0012-1606
VL - 187
SP - 224
EP - 235
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -