Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium

N. A. Buchmeier, Fred Heffron

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Salmonella typhimurium-infected macrophages were examined by electron microscopy to determine whether intracellular survival of S. typhimurium is associated with failure of bacteria containing phagosomes to fuse with secondary lysosomes. S. typhimurium 14028 actively inhibited phagosome-lysosome fusion and appeared to preferentially divide within unfused phagocytic vesicles. In comparison with Escherichia coli, S. typhimurium inhibited phagosome-lysosome fusion in peritoneal macrophages, J774 macrophages, and bone marrow derived macrophages from both BALB/c (ity(s)) and SWR/J (ity(r)) mice. The mechanism responsible for Salmonella inhibition of phagosome-lysosome fusion is unknown but requires viable salmonellae, is not blocked by opsonization with fresh normal mouse serum, and is not due to lipopolysaccharide. Inhibition of phagosome-lysosome fusion may play a critical role in survival of salmonellae within macrophages and in virulence.

Original languageEnglish (US)
Pages (from-to)2232-2238
Number of pages7
JournalInfection and Immunity
Volume59
Issue number7
StatePublished - 1991
Externally publishedYes

Fingerprint

Phagosomes
Salmonella typhimurium
Lysosomes
Macrophages
Salmonella
Peritoneal Macrophages
Virulence
Lipopolysaccharides
Electron Microscopy
Escherichia coli
Bacteria
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium. / Buchmeier, N. A.; Heffron, Fred.

In: Infection and Immunity, Vol. 59, No. 7, 1991, p. 2232-2238.

Research output: Contribution to journalArticle

Buchmeier, NA & Heffron, F 1991, 'Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium', Infection and Immunity, vol. 59, no. 7, pp. 2232-2238.
Buchmeier, N. A. ; Heffron, Fred. / Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium. In: Infection and Immunity. 1991 ; Vol. 59, No. 7. pp. 2232-2238.
@article{8f34be70887449c3ab76240e9c8d1d00,
title = "Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium",
abstract = "Salmonella typhimurium-infected macrophages were examined by electron microscopy to determine whether intracellular survival of S. typhimurium is associated with failure of bacteria containing phagosomes to fuse with secondary lysosomes. S. typhimurium 14028 actively inhibited phagosome-lysosome fusion and appeared to preferentially divide within unfused phagocytic vesicles. In comparison with Escherichia coli, S. typhimurium inhibited phagosome-lysosome fusion in peritoneal macrophages, J774 macrophages, and bone marrow derived macrophages from both BALB/c (ity(s)) and SWR/J (ity(r)) mice. The mechanism responsible for Salmonella inhibition of phagosome-lysosome fusion is unknown but requires viable salmonellae, is not blocked by opsonization with fresh normal mouse serum, and is not due to lipopolysaccharide. Inhibition of phagosome-lysosome fusion may play a critical role in survival of salmonellae within macrophages and in virulence.",
author = "Buchmeier, {N. A.} and Fred Heffron",
year = "1991",
language = "English (US)",
volume = "59",
pages = "2232--2238",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "7",

}

TY - JOUR

T1 - Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium

AU - Buchmeier, N. A.

AU - Heffron, Fred

PY - 1991

Y1 - 1991

N2 - Salmonella typhimurium-infected macrophages were examined by electron microscopy to determine whether intracellular survival of S. typhimurium is associated with failure of bacteria containing phagosomes to fuse with secondary lysosomes. S. typhimurium 14028 actively inhibited phagosome-lysosome fusion and appeared to preferentially divide within unfused phagocytic vesicles. In comparison with Escherichia coli, S. typhimurium inhibited phagosome-lysosome fusion in peritoneal macrophages, J774 macrophages, and bone marrow derived macrophages from both BALB/c (ity(s)) and SWR/J (ity(r)) mice. The mechanism responsible for Salmonella inhibition of phagosome-lysosome fusion is unknown but requires viable salmonellae, is not blocked by opsonization with fresh normal mouse serum, and is not due to lipopolysaccharide. Inhibition of phagosome-lysosome fusion may play a critical role in survival of salmonellae within macrophages and in virulence.

AB - Salmonella typhimurium-infected macrophages were examined by electron microscopy to determine whether intracellular survival of S. typhimurium is associated with failure of bacteria containing phagosomes to fuse with secondary lysosomes. S. typhimurium 14028 actively inhibited phagosome-lysosome fusion and appeared to preferentially divide within unfused phagocytic vesicles. In comparison with Escherichia coli, S. typhimurium inhibited phagosome-lysosome fusion in peritoneal macrophages, J774 macrophages, and bone marrow derived macrophages from both BALB/c (ity(s)) and SWR/J (ity(r)) mice. The mechanism responsible for Salmonella inhibition of phagosome-lysosome fusion is unknown but requires viable salmonellae, is not blocked by opsonization with fresh normal mouse serum, and is not due to lipopolysaccharide. Inhibition of phagosome-lysosome fusion may play a critical role in survival of salmonellae within macrophages and in virulence.

UR - http://www.scopus.com/inward/record.url?scp=0025830323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025830323&partnerID=8YFLogxK

M3 - Article

C2 - 2050395

AN - SCOPUS:0025830323

VL - 59

SP - 2232

EP - 2238

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 7

ER -