Inhibition of ca2+ uptake into A7r5 vascular smooth muscle cells by farnesol: Lack of effect on membrane fluidity and Ca2+‐ATPase activities

Jean Baptiste Roullet, Kim H.Le Quan Sang, Ulrich Luft, Mitsuake Watanabe, Keiichi Otsuka, David A. McCarron, Marie Aude Devynck

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Previous studies have shown that farnesol, a 15-carbon nonsterol derivative of mevalonic acid, inhibits vasoconstriction. Because of its lipophilic properties, we hypothesized that farnesol increased membrane dynamics, thus reducing uptake of Ca2+ and contraction. To characterize the effect of farnesol on cell membrane fluidity. The study was conducted using A7r5 cells, a rat aortic vascular smooth muscle cell line. Inhibition of Ca2+ uptake by farnesol was first established in these cells. Then, the effect of farnesol on membrane dynamics was determined. Finally, to ascertain that activation of Ca2+ extrusion and reuptake processes by farnesol did not occur, Ca2+ -ATPase activity was examined.

Original languageEnglish (US)
Pages (from-to)1723-1728
Number of pages6
JournalJournal of Hypertension
Volume15
Issue number12
DOIs
StatePublished - Dec 1997

Keywords

  • Ca-ATPase
  • Farnesol
  • Isoprenoids
  • Membrane fluidity
  • Smooth muscle cells

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

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