Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly

Megan Troxell, Shobha Gopalakrishnan, Joanne McCormack, Bradley A. Poteat, Janice Pennington, Sean M. Garringer, Eveline E. Schneeberger, W. James Nelson, James A. Marrs

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Previous studies have shown that induction of cadherin-mediated cell-cell adhesion leads to tight junction formation, and that blocking cadherin-mediated cell-cell adhesion inhibits tight junction assembly. Here we report analysis of tight junction assembly in MDCK cells overexpressing a mutant E-cadherin protein that lacks an adhesive extracellular domain (T151 cells). Mutant E-cadherin overexpression caused a dramatic reduction in endogenous cadherin levels. Despite this, tight junction assembly was extensive. The number of tight junction strands observed by freeze-fracture electron microscopy significantly increased in T151 cells compared to that in control cells. Our data indicate that the hierarchical regulation of junctional complex assembly is not absolute, and that inhibition of cadherin function has both positive and negative effects on tight junction assembly.

Original languageEnglish (US)
Pages (from-to)985-996
Number of pages12
JournalJournal of Cell Science
Volume113
Issue number6
StatePublished - 2000
Externally publishedYes

Fingerprint

Tight Junctions
Cadherins
Cell Adhesion
Madin Darby Canine Kidney Cells
Adhesives
Electron Microscopy
Proteins

Keywords

  • Adherens junction
  • E-cadherin
  • Tight junction

ASJC Scopus subject areas

  • Cell Biology

Cite this

Troxell, M., Gopalakrishnan, S., McCormack, J., Poteat, B. A., Pennington, J., Garringer, S. M., ... Marrs, J. A. (2000). Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly. Journal of Cell Science, 113(6), 985-996.

Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly. / Troxell, Megan; Gopalakrishnan, Shobha; McCormack, Joanne; Poteat, Bradley A.; Pennington, Janice; Garringer, Sean M.; Schneeberger, Eveline E.; Nelson, W. James; Marrs, James A.

In: Journal of Cell Science, Vol. 113, No. 6, 2000, p. 985-996.

Research output: Contribution to journalArticle

Troxell, M, Gopalakrishnan, S, McCormack, J, Poteat, BA, Pennington, J, Garringer, SM, Schneeberger, EE, Nelson, WJ & Marrs, JA 2000, 'Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly', Journal of Cell Science, vol. 113, no. 6, pp. 985-996.
Troxell M, Gopalakrishnan S, McCormack J, Poteat BA, Pennington J, Garringer SM et al. Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly. Journal of Cell Science. 2000;113(6):985-996.
Troxell, Megan ; Gopalakrishnan, Shobha ; McCormack, Joanne ; Poteat, Bradley A. ; Pennington, Janice ; Garringer, Sean M. ; Schneeberger, Eveline E. ; Nelson, W. James ; Marrs, James A. / Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly. In: Journal of Cell Science. 2000 ; Vol. 113, No. 6. pp. 985-996.
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