Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma

A experimental study

Xin Li, Gan Sheng Feng, Chuan Sheng Zheng, Chen Kai Zhuo, Xi Liu

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Aim: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive responses. For example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted hepatoma. Methods: Male Wistar rats were inoculated with Walker-256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-Fu, 5-Fu and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor VIII, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density (MVD) was counted in endothelial cells with positive factor VIII. Their expression levels were analyzed in conjunction with the pathologic features. Results: While a smaller tumor volume was found in TACE group (F=37.818, P2=0.449, P=0.799) and 30 %, 25 %, 30 % (Χ2=0.141, P=0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r=0.552, P

Original languageEnglish (US)
Pages (from-to)2445-2449
Number of pages5
JournalWorld Journal of Gastroenterology
Volume9
Issue number11
StatePublished - Nov 2003
Externally publishedYes

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Walkers
Experimental Liver Neoplasms
Fibroblast Growth Factor 2
Vascular Endothelial Growth Factor A
Factor VIII
Microvessels
Hepatocellular Carcinoma
Ethiodized Oil
Hepatic Artery
Residual Neoplasm
Tumor Burden
Paraffin
Wistar Rats
Angiography
Endothelial Cells
Transplantation
Immunohistochemistry
Staining and Labeling
Control Groups
Liver

ASJC Scopus subject areas

  • Gastroenterology

Cite this

@article{e07f7cf5008f4b5aa90eadd83b0bb709,
title = "Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma: A experimental study",
abstract = "Aim: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive responses. For example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted hepatoma. Methods: Male Wistar rats were inoculated with Walker-256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-Fu, 5-Fu and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor VIII, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density (MVD) was counted in endothelial cells with positive factor VIII. Their expression levels were analyzed in conjunction with the pathologic features. Results: While a smaller tumor volume was found in TACE group (F=37.818, P2=0.449, P=0.799) and 30 {\%}, 25 {\%}, 30 {\%} (Χ2=0.141, P=0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r=0.552, P",
author = "Xin Li and Feng, {Gan Sheng} and Zheng, {Chuan Sheng} and Zhuo, {Chen Kai} and Xi Liu",
year = "2003",
month = "11",
language = "English (US)",
volume = "9",
pages = "2445--2449",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
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number = "11",

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TY - JOUR

T1 - Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma

T2 - A experimental study

AU - Li, Xin

AU - Feng, Gan Sheng

AU - Zheng, Chuan Sheng

AU - Zhuo, Chen Kai

AU - Liu, Xi

PY - 2003/11

Y1 - 2003/11

N2 - Aim: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive responses. For example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted hepatoma. Methods: Male Wistar rats were inoculated with Walker-256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-Fu, 5-Fu and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor VIII, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density (MVD) was counted in endothelial cells with positive factor VIII. Their expression levels were analyzed in conjunction with the pathologic features. Results: While a smaller tumor volume was found in TACE group (F=37.818, P2=0.449, P=0.799) and 30 %, 25 %, 30 % (Χ2=0.141, P=0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r=0.552, P

AB - Aim: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive responses. For example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted hepatoma. Methods: Male Wistar rats were inoculated with Walker-256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-Fu, 5-Fu and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor VIII, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density (MVD) was counted in endothelial cells with positive factor VIII. Their expression levels were analyzed in conjunction with the pathologic features. Results: While a smaller tumor volume was found in TACE group (F=37.818, P2=0.449, P=0.799) and 30 %, 25 %, 30 % (Χ2=0.141, P=0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r=0.552, P

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