Influence of intense sound exposure on glutathione synthesis in the cochlea

Tatsuya Yamasoba, Craig Harris, Fumi Shoji, Rosanna J. Lee, Alfred Nuttall, Josef M. Miller

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Previous studies have shown that depletion of endogenous glutathione (GSH) potentiates noise-induced hearing loss (NIHL), whereas replenishment of GSH attenuates NIHL (Yamasoba et al., Brain Res. 784 (1998) 82-90). Since these findings indicate an important role of GSH in protection from NIHL, we assessed the influence of intense sound exposure (broadband noise, 105 dB SPL, 5 h) on GSH and cysteine levels in the guinea pig cochlea using high performance liquid chromatography. GSH levels were significantly increased in the lateral wall 2 and 4 h post-exposure and returned to normal 6 h post- exposure. GSH levels in the sensory epithelium and modiolus did not show significant changes following noise. Cysteine levels were unchanged in any of the cochlear segments. For the cochlea as a whole, intense sound exposure did not significantly change GSH or cysteine levels throughout the 6-h measurement period post-exposure. These results indicate that GSH synthesis is markedly upregulated selectively in the lateral wall by noise exposure, presumably in response to the robust consumption of GSH, as it is utilized in scavenging reactive oxygen species.

Original languageEnglish (US)
Pages (from-to)72-78
Number of pages7
JournalBrain Research
Volume804
Issue number1
DOIs
StatePublished - Aug 31 1998

Fingerprint

Noise-Induced Hearing Loss
Cochlea
Glutathione
Cysteine
Noise
Reactive Oxygen Species
Guinea Pigs
Epithelium
High Pressure Liquid Chromatography
Brain

Keywords

  • Acoustic trauma
  • Cochlea
  • Guinea pig
  • High- performance liquid chromatography
  • Noise-induced hearing loss
  • Reactive oxygen species

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Influence of intense sound exposure on glutathione synthesis in the cochlea. / Yamasoba, Tatsuya; Harris, Craig; Shoji, Fumi; Lee, Rosanna J.; Nuttall, Alfred; Miller, Josef M.

In: Brain Research, Vol. 804, No. 1, 31.08.1998, p. 72-78.

Research output: Contribution to journalArticle

Yamasoba, Tatsuya ; Harris, Craig ; Shoji, Fumi ; Lee, Rosanna J. ; Nuttall, Alfred ; Miller, Josef M. / Influence of intense sound exposure on glutathione synthesis in the cochlea. In: Brain Research. 1998 ; Vol. 804, No. 1. pp. 72-78.
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