Influence of high fat diet and resveratrol supplementation on placental fatty acid uptake in the Japanese macaque

P. O'Tierney-Ginn, Victoria Roberts, Melanie Gillingham, J. Walker, P. A. Glazebrook, Kent Thornburg, Kevin Grove, Antonio Frias

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Introduction Adequate maternal supply and placental delivery of long chain polyunsaturated fatty acids (LCPUFA) is essential for normal fetal development. In humans, maternal obesity alters placental FA uptake, though the impact of diet remains uncertain. The fatty fetal liver observed in offspring of Japanese macaques fed a high fat diet (HFD) was prevented with resveratrol supplementation during pregnancy. We sought to determine the effect of HFD and resveratrol, a supplement with insulin-sensitizing properties, on placental LCPUFA uptake in this model. Methods J. macaques were fed control chow (15% fat, n = 5), HFD (35% fat, n = 10) or HFD containing 0.37% resveratrol (n = 5) prior to- and throughout pregnancy. At ∼130d gestation (term = 173d), placentas were collected by caesarean section. Fatty acid uptake studies using 14C-labeled oleic acid, arachidonic acid (AA) and docosahexanoic acid (DHA) were performed in placental explants. Results Resveratrol supplementation increased placental uptake of DHA (P <0.05), while HFD alone had no measurable effect. Resveratrol increased AMP-activated protein kinase activity and mRNA expression of the fatty acid transporters FATP-4, CD36 and FABPpm (P <0.05). Placental DHA content was decreased in HFD dams; resveratrol had no effect on tissue fatty acid profiles. Discussion Maternal HFD did not significantly affect placental LCPUFA uptake. Furthermore, resveratrol stimulated placental DHA uptake capacity, AMPK activation and transporter expression. Placental handling of DHA is particularly sensitive to the dramatic alterations in the maternal metabolic phenotype and placental AMPK activity associated with resveratrol supplementation.

Original languageEnglish (US)
Pages (from-to)903-910
Number of pages8
JournalPlacenta
Volume36
Issue number8
DOIs
StatePublished - Aug 1 2015

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Macaca
High Fat Diet
Fatty Acids
AMP-Activated Protein Kinases
Acids
Unsaturated Fatty Acids
Mothers
Pregnancy
Fats
resveratrol
Fatty Liver
Oleic Acid
Fetal Development
Arachidonic Acid
Cesarean Section
Placenta
Obesity
Insulin
Diet
Phenotype

Keywords

  • Fatty acid uptake
  • High fat diet
  • Non-human primate
  • Placenta

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Influence of high fat diet and resveratrol supplementation on placental fatty acid uptake in the Japanese macaque. / O'Tierney-Ginn, P.; Roberts, Victoria; Gillingham, Melanie; Walker, J.; Glazebrook, P. A.; Thornburg, Kent; Grove, Kevin; Frias, Antonio.

In: Placenta, Vol. 36, No. 8, 01.08.2015, p. 903-910.

Research output: Contribution to journalArticle

O'Tierney-Ginn, P. ; Roberts, Victoria ; Gillingham, Melanie ; Walker, J. ; Glazebrook, P. A. ; Thornburg, Kent ; Grove, Kevin ; Frias, Antonio. / Influence of high fat diet and resveratrol supplementation on placental fatty acid uptake in the Japanese macaque. In: Placenta. 2015 ; Vol. 36, No. 8. pp. 903-910.
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abstract = "Introduction Adequate maternal supply and placental delivery of long chain polyunsaturated fatty acids (LCPUFA) is essential for normal fetal development. In humans, maternal obesity alters placental FA uptake, though the impact of diet remains uncertain. The fatty fetal liver observed in offspring of Japanese macaques fed a high fat diet (HFD) was prevented with resveratrol supplementation during pregnancy. We sought to determine the effect of HFD and resveratrol, a supplement with insulin-sensitizing properties, on placental LCPUFA uptake in this model. Methods J. macaques were fed control chow (15{\%} fat, n = 5), HFD (35{\%} fat, n = 10) or HFD containing 0.37{\%} resveratrol (n = 5) prior to- and throughout pregnancy. At ∼130d gestation (term = 173d), placentas were collected by caesarean section. Fatty acid uptake studies using 14C-labeled oleic acid, arachidonic acid (AA) and docosahexanoic acid (DHA) were performed in placental explants. Results Resveratrol supplementation increased placental uptake of DHA (P <0.05), while HFD alone had no measurable effect. Resveratrol increased AMP-activated protein kinase activity and mRNA expression of the fatty acid transporters FATP-4, CD36 and FABPpm (P <0.05). Placental DHA content was decreased in HFD dams; resveratrol had no effect on tissue fatty acid profiles. Discussion Maternal HFD did not significantly affect placental LCPUFA uptake. Furthermore, resveratrol stimulated placental DHA uptake capacity, AMPK activation and transporter expression. Placental handling of DHA is particularly sensitive to the dramatic alterations in the maternal metabolic phenotype and placental AMPK activity associated with resveratrol supplementation.",
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AU - Walker, J.

AU - Glazebrook, P. A.

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AU - Grove, Kevin

AU - Frias, Antonio

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N2 - Introduction Adequate maternal supply and placental delivery of long chain polyunsaturated fatty acids (LCPUFA) is essential for normal fetal development. In humans, maternal obesity alters placental FA uptake, though the impact of diet remains uncertain. The fatty fetal liver observed in offspring of Japanese macaques fed a high fat diet (HFD) was prevented with resveratrol supplementation during pregnancy. We sought to determine the effect of HFD and resveratrol, a supplement with insulin-sensitizing properties, on placental LCPUFA uptake in this model. Methods J. macaques were fed control chow (15% fat, n = 5), HFD (35% fat, n = 10) or HFD containing 0.37% resveratrol (n = 5) prior to- and throughout pregnancy. At ∼130d gestation (term = 173d), placentas were collected by caesarean section. Fatty acid uptake studies using 14C-labeled oleic acid, arachidonic acid (AA) and docosahexanoic acid (DHA) were performed in placental explants. Results Resveratrol supplementation increased placental uptake of DHA (P <0.05), while HFD alone had no measurable effect. Resveratrol increased AMP-activated protein kinase activity and mRNA expression of the fatty acid transporters FATP-4, CD36 and FABPpm (P <0.05). Placental DHA content was decreased in HFD dams; resveratrol had no effect on tissue fatty acid profiles. Discussion Maternal HFD did not significantly affect placental LCPUFA uptake. Furthermore, resveratrol stimulated placental DHA uptake capacity, AMPK activation and transporter expression. Placental handling of DHA is particularly sensitive to the dramatic alterations in the maternal metabolic phenotype and placental AMPK activity associated with resveratrol supplementation.

AB - Introduction Adequate maternal supply and placental delivery of long chain polyunsaturated fatty acids (LCPUFA) is essential for normal fetal development. In humans, maternal obesity alters placental FA uptake, though the impact of diet remains uncertain. The fatty fetal liver observed in offspring of Japanese macaques fed a high fat diet (HFD) was prevented with resveratrol supplementation during pregnancy. We sought to determine the effect of HFD and resveratrol, a supplement with insulin-sensitizing properties, on placental LCPUFA uptake in this model. Methods J. macaques were fed control chow (15% fat, n = 5), HFD (35% fat, n = 10) or HFD containing 0.37% resveratrol (n = 5) prior to- and throughout pregnancy. At ∼130d gestation (term = 173d), placentas were collected by caesarean section. Fatty acid uptake studies using 14C-labeled oleic acid, arachidonic acid (AA) and docosahexanoic acid (DHA) were performed in placental explants. Results Resveratrol supplementation increased placental uptake of DHA (P <0.05), while HFD alone had no measurable effect. Resveratrol increased AMP-activated protein kinase activity and mRNA expression of the fatty acid transporters FATP-4, CD36 and FABPpm (P <0.05). Placental DHA content was decreased in HFD dams; resveratrol had no effect on tissue fatty acid profiles. Discussion Maternal HFD did not significantly affect placental LCPUFA uptake. Furthermore, resveratrol stimulated placental DHA uptake capacity, AMPK activation and transporter expression. Placental handling of DHA is particularly sensitive to the dramatic alterations in the maternal metabolic phenotype and placental AMPK activity associated with resveratrol supplementation.

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