Influence of biofilm formation on the optical properties of novel bioactive glass-containing composites

Hong Keun Hyun, Jack Ferracane

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective Bioactive glass (BAG) has been suggested as a possible additive for dental restorative materials because of its antimicrobial effect and potential for promoting apatite formation in body fluids. The purpose of this study was to investigate the effects of bacterial biofilm on the change of colorimetric value and translucency of novel BAG-containing composites having different initial surface roughness. Methods Composites with 72 wt% total filler load were prepared by replacing 15% of the silanized Sr glass with BAG (65 mol % Si; 4% P; 31% Ca), BAG-F (61% Si; 31% Ca; 4% P; 3% F; 1% B), or silanized silica. Light-cured discs of 2-mm thickness (n = 10/group) were divided into 4 different surface roughness subgroups produced by wet polishing with 600 and then up to 1200, 2400, or 4000 grit SiC. CIE L*a*b* were measured and the color difference and translucency parameter (TP) were calculated before and after incubating in media with or without a Streptococcus mutans (UA 159) biofilm for 2 wks (no agitation). Results were analyzed using ANOVA/Tukey's test (α = 0.05). Results All the color differences for BAG and BAG-F composite showed significant decreases with bacterial biofilm compared to media-only. The mean TP (SD) of BAG and BAG-F composite before aging [10.0 (2.8) and 8.5 (1.4)] was higher than that of the control composite [4.9 (0.8)], while the change in TP with aging was greater compared to the control with or without bacteria. BAG-F composites with the smoothest surfaces showed a greater decrease in TP under bacterial biofilm compared to the BAG composite. Significance Highly polished dental composites containing bioactive glass additives may become slightly rougher and show reduced translucency when exposed to bacterial biofilms, but do not discolor any more than control composites that do not contain the BAG.

Original languageEnglish (US)
Pages (from-to)1144-1151
Number of pages8
JournalDental Materials
Volume32
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Bioactive glass
Biofilms
Glass
Optical properties
Composite materials
Aging of materials
Surface roughness
Dental composites
Color
Dental materials
Apatites
Dental Materials
Body fluids
Streptococcus mutans
Apatite
Analysis of variance (ANOVA)
Polishing
Body Fluids
Silicon Dioxide
Fillers

Keywords

  • Bioactive glass
  • Biofilms
  • Color
  • Dental composites

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Influence of biofilm formation on the optical properties of novel bioactive glass-containing composites. / Hyun, Hong Keun; Ferracane, Jack.

In: Dental Materials, Vol. 32, No. 9, 01.09.2016, p. 1144-1151.

Research output: Contribution to journalArticle

@article{adc5a00a7bac42caa260255a01211be2,
title = "Influence of biofilm formation on the optical properties of novel bioactive glass-containing composites",
abstract = "Objective Bioactive glass (BAG) has been suggested as a possible additive for dental restorative materials because of its antimicrobial effect and potential for promoting apatite formation in body fluids. The purpose of this study was to investigate the effects of bacterial biofilm on the change of colorimetric value and translucency of novel BAG-containing composites having different initial surface roughness. Methods Composites with 72 wt{\%} total filler load were prepared by replacing 15{\%} of the silanized Sr glass with BAG (65 mol {\%} Si; 4{\%} P; 31{\%} Ca), BAG-F (61{\%} Si; 31{\%} Ca; 4{\%} P; 3{\%} F; 1{\%} B), or silanized silica. Light-cured discs of 2-mm thickness (n = 10/group) were divided into 4 different surface roughness subgroups produced by wet polishing with 600 and then up to 1200, 2400, or 4000 grit SiC. CIE L*a*b* were measured and the color difference and translucency parameter (TP) were calculated before and after incubating in media with or without a Streptococcus mutans (UA 159) biofilm for 2 wks (no agitation). Results were analyzed using ANOVA/Tukey's test (α = 0.05). Results All the color differences for BAG and BAG-F composite showed significant decreases with bacterial biofilm compared to media-only. The mean TP (SD) of BAG and BAG-F composite before aging [10.0 (2.8) and 8.5 (1.4)] was higher than that of the control composite [4.9 (0.8)], while the change in TP with aging was greater compared to the control with or without bacteria. BAG-F composites with the smoothest surfaces showed a greater decrease in TP under bacterial biofilm compared to the BAG composite. Significance Highly polished dental composites containing bioactive glass additives may become slightly rougher and show reduced translucency when exposed to bacterial biofilms, but do not discolor any more than control composites that do not contain the BAG.",
keywords = "Bioactive glass, Biofilms, Color, Dental composites",
author = "Hyun, {Hong Keun} and Jack Ferracane",
year = "2016",
month = "9",
day = "1",
doi = "10.1016/j.dental.2016.06.011",
language = "English (US)",
volume = "32",
pages = "1144--1151",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier Science",
number = "9",

}

TY - JOUR

T1 - Influence of biofilm formation on the optical properties of novel bioactive glass-containing composites

AU - Hyun, Hong Keun

AU - Ferracane, Jack

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Objective Bioactive glass (BAG) has been suggested as a possible additive for dental restorative materials because of its antimicrobial effect and potential for promoting apatite formation in body fluids. The purpose of this study was to investigate the effects of bacterial biofilm on the change of colorimetric value and translucency of novel BAG-containing composites having different initial surface roughness. Methods Composites with 72 wt% total filler load were prepared by replacing 15% of the silanized Sr glass with BAG (65 mol % Si; 4% P; 31% Ca), BAG-F (61% Si; 31% Ca; 4% P; 3% F; 1% B), or silanized silica. Light-cured discs of 2-mm thickness (n = 10/group) were divided into 4 different surface roughness subgroups produced by wet polishing with 600 and then up to 1200, 2400, or 4000 grit SiC. CIE L*a*b* were measured and the color difference and translucency parameter (TP) were calculated before and after incubating in media with or without a Streptococcus mutans (UA 159) biofilm for 2 wks (no agitation). Results were analyzed using ANOVA/Tukey's test (α = 0.05). Results All the color differences for BAG and BAG-F composite showed significant decreases with bacterial biofilm compared to media-only. The mean TP (SD) of BAG and BAG-F composite before aging [10.0 (2.8) and 8.5 (1.4)] was higher than that of the control composite [4.9 (0.8)], while the change in TP with aging was greater compared to the control with or without bacteria. BAG-F composites with the smoothest surfaces showed a greater decrease in TP under bacterial biofilm compared to the BAG composite. Significance Highly polished dental composites containing bioactive glass additives may become slightly rougher and show reduced translucency when exposed to bacterial biofilms, but do not discolor any more than control composites that do not contain the BAG.

AB - Objective Bioactive glass (BAG) has been suggested as a possible additive for dental restorative materials because of its antimicrobial effect and potential for promoting apatite formation in body fluids. The purpose of this study was to investigate the effects of bacterial biofilm on the change of colorimetric value and translucency of novel BAG-containing composites having different initial surface roughness. Methods Composites with 72 wt% total filler load were prepared by replacing 15% of the silanized Sr glass with BAG (65 mol % Si; 4% P; 31% Ca), BAG-F (61% Si; 31% Ca; 4% P; 3% F; 1% B), or silanized silica. Light-cured discs of 2-mm thickness (n = 10/group) were divided into 4 different surface roughness subgroups produced by wet polishing with 600 and then up to 1200, 2400, or 4000 grit SiC. CIE L*a*b* were measured and the color difference and translucency parameter (TP) were calculated before and after incubating in media with or without a Streptococcus mutans (UA 159) biofilm for 2 wks (no agitation). Results were analyzed using ANOVA/Tukey's test (α = 0.05). Results All the color differences for BAG and BAG-F composite showed significant decreases with bacterial biofilm compared to media-only. The mean TP (SD) of BAG and BAG-F composite before aging [10.0 (2.8) and 8.5 (1.4)] was higher than that of the control composite [4.9 (0.8)], while the change in TP with aging was greater compared to the control with or without bacteria. BAG-F composites with the smoothest surfaces showed a greater decrease in TP under bacterial biofilm compared to the BAG composite. Significance Highly polished dental composites containing bioactive glass additives may become slightly rougher and show reduced translucency when exposed to bacterial biofilms, but do not discolor any more than control composites that do not contain the BAG.

KW - Bioactive glass

KW - Biofilms

KW - Color

KW - Dental composites

UR - http://www.scopus.com/inward/record.url?scp=84990857117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990857117&partnerID=8YFLogxK

U2 - 10.1016/j.dental.2016.06.011

DO - 10.1016/j.dental.2016.06.011

M3 - Article

C2 - 27394086

AN - SCOPUS:84990857117

VL - 32

SP - 1144

EP - 1151

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

IS - 9

ER -