Influence of angiotensin on total intravascular capacity in the anaesthetized pig

The present study examined the influence of angiotensin on total intravascular capacity. In eight anaesthetized pigs, splenectomy, carotid sinus denervation and cervical vagotomy were performed. Blood was drained from the venae cavae to an extracorporeal reservoir and returned to the right atrium at a constant rate so that changes in total intravascular volume could be recorded as the inverse of changes in reservoir volume. Angiotensin administration at 0.2 μg kg^-1 min^-1 i.v. for 5 min was associated with a decrease in total intravascular volume of 57 ± 6 ml (P < 0.05) and an increase in aortic pressure from 96 ± 5 to 119 ± 6 mmHg (P < 0.05). With subsequent angiotensin administration in five of the animals, the responses were not attenuated. In five of the animals, angiotensin was associated with a decrease in intravascular volume of 72 ± 8 ml (P < 0.05) before abdominal evisceration and 33 ± 13 ml (P < 0.05) after evisceration. These responses were significantly different from each other. In four of these eviscerated animals, angiotensin was associated with a decrease in intravascular volume of 35 ± 17 ml (P < 0.05) before ligation of all four limbs and a decrease of 36 ± 4 ml (P < 0.05) after limb ligation. Thus, angiotensin acts directly to decrease total intravascular volume. The decrease is due to decreases in both splanchnic and extrasplanchnic volume. The extrasplanchnic volume decrement is not due to decreases in skeletal muscle or cutaneous tissue intravascular capacity in the limbs.