Influence of a model human defensive peroxidase system on oral streptococcal antagonism

Michael T. Ashby, Jens Kreth, Muthu Soundarajan, Laure Sita Sivuilu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Streptococcus is a dominant genus in the human oral cavity, making up about 20% of the more than 800 species of bacteria that have been identified, and about 80% of the early biofilm colonizers. Oral streptococci include both health-compatible (e.g. Streptococcus gordonii and Streptococcus sanguinis) and pathogenic strains (e.g. the cariogenic Streptococcus mutans). Because the streptococci have similar metabolic requirements, they have developed defence strategies that lead to antagonism (also known as bacterial interference). S. mutans expresses bacteriocins that are cytotoxic toward S. gordonii and S. sanguinis, whereas S. gordonii and S. sanguinis differentially produce H2O2 (under aerobic growth conditions), which is relatively toxic toward S. mutans. Superimposed on the inter-bacterial combat are the effects of the host defensive mechanisms. We report here on the multifarious effects of bovine lactoperoxidase (bLPO) on the antagonism between S. gordonii and S. sanguinis versus S. mutans. Some of the effects are apparently counterproductive with respect to maintaining a health-compatible population of streptococci. For example, the bLPO system (comprised of bLPO+SCN-+H2O2) destroys H2O2, thereby abolishing the ability of S. gordonii and S. sanguinis to inhibit the growth of S. mutans. Furthermore, bLPO protein (with or without its substrate) inhibits bacterial growth in a biofilm assay, but sucrose negates the inhibitory effects of the bLPO protein, thereby facilitating adherence of S. mutans in lieu of S. gordonii and S. sanguinis. Our findings may be relevant to environmental pressures that select early supragingival colonizers.

Original languageEnglish (US)
Pages (from-to)3691-3700
Number of pages10
JournalMicrobiology
Volume155
Issue number11
DOIs
StatePublished - Nov 30 2009
Externally publishedYes

Fingerprint

Streptococcus gordonii
Streptococcus mutans
Lactoperoxidase
Peroxidase
Streptococcus
Biofilms
Growth
Antibiosis
Bacteriocins
Poisons
Health
Sucrose
Mouth
Proteins
Bacteria
Pressure

ASJC Scopus subject areas

  • Microbiology

Cite this

Influence of a model human defensive peroxidase system on oral streptococcal antagonism. / Ashby, Michael T.; Kreth, Jens; Soundarajan, Muthu; Sivuilu, Laure Sita.

In: Microbiology, Vol. 155, No. 11, 30.11.2009, p. 3691-3700.

Research output: Contribution to journalArticle

Ashby, Michael T. ; Kreth, Jens ; Soundarajan, Muthu ; Sivuilu, Laure Sita. / Influence of a model human defensive peroxidase system on oral streptococcal antagonism. In: Microbiology. 2009 ; Vol. 155, No. 11. pp. 3691-3700.
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