Inflammation and the emerging role of the toll-like receptor system in acute brain ischemia

Brenda J. Marsh, Susan L. Stevens, Brian Hunter, Mary Stenzel-Poore

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background and Purpose-Systemic administration of cytosine-guanine (CpG) oligodeoxynucleotides provides neuroprotection against subsequent cerebral ischemic injury. We examined the genomic response of leukocytes and brain cells after ischemia in the context of CpG preconditioning. Methods-RNA was isolated from circulating leukocytes and ischemic cortex 3 and 24 hours after middle cerebral artery occlusion after CpG or saline pretreatment and subjected to microarray analysis. Genes uniquely upregulated in CpG-pretreated mice were examined for overrepresented transcriptional regulatory elements. Results-CpG preconditioning induced a novel response to middle cerebral artery occlusion within circulating leukocytes that was dominated by natural killer cell-associated genes and the GATA-3 transcriptional regulatory element. Preconditioning also caused a novel brain response to stroke that was dominated by Type I interferon, interferon-associated genes, and transcriptional regulatory elements. Conclusion-CpG preconditioning invokes novel leukocyte and brain responses to stroke. In this, CpG may be a unique preconditioning agent, coordinating peripheral and brain responses to protect against ischemic injury.

Original languageEnglish (US)
JournalStroke
Volume40
Issue number3 SUPPL. 1
DOIs
StatePublished - Mar 2009

Fingerprint

Transcriptional Regulatory Elements
Toll-Like Receptors
Brain Ischemia
Leukocytes
Inflammation
Middle Cerebral Artery Infarction
Brain
Stroke
Genes
Interferon Type I
Oligodeoxyribonucleotides
Cytosine
Wounds and Injuries
Guanine
Microarray Analysis
Natural Killer Cells
Interferons
Ischemia
RNA

Keywords

  • Hypoxia-ischemia
  • Inflammation
  • Stroke

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing

Cite this

Inflammation and the emerging role of the toll-like receptor system in acute brain ischemia. / Marsh, Brenda J.; Stevens, Susan L.; Hunter, Brian; Stenzel-Poore, Mary.

In: Stroke, Vol. 40, No. 3 SUPPL. 1, 03.2009.

Research output: Contribution to journalArticle

Marsh, Brenda J. ; Stevens, Susan L. ; Hunter, Brian ; Stenzel-Poore, Mary. / Inflammation and the emerging role of the toll-like receptor system in acute brain ischemia. In: Stroke. 2009 ; Vol. 40, No. 3 SUPPL. 1.
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