Infection with a molecularly cloned SIVsm virus elicits high titer homologous neutralizing antibodies with heterologous neutralizing activity

M. Mahalanabis, V. M. Hirsch, Nancy L. Haigwood

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We have evaluated the homologous and heterologous neutralizing antibody response in a cohort of six Macaca nemestrina infected with the cloned virus SIVsm62d that showed different levels of envelope diversification. Two progressor macaques developed AIDS by 1.5 years post-inoculation and four non-progressors were asymptomatic for 3 years of follow-up. All macaques developed high titers of neutralizing antibodies against homologous SIVsm viruses and intermediate titers against SIVsmB670. Heterologous virus neutralization of SIVmac, SIVmne, and HIV-2 was detected at much lower levels in both progressor macaques; only one of four non-progressors had evidence for broader neutralizing antibody activity. We noted changes in potential N-linked glycosylation (PNG) sites in V1/V2, C2, and V4 that were common to multiple macaques. These results support a model for viral neutralization where heterologous neutralization is, in part, driven by a strong homologous response and may be coupled to changes in PNG sites in envelop.

Original languageEnglish (US)
Pages (from-to)253-261
Number of pages9
JournalJournal of medical primatology
Volume34
Issue number5-6 SPEC. ISS.
DOIs
StatePublished - Oct 2005
Externally publishedYes

Keywords

  • Broad neutralization
  • N-linked glycosylation
  • Simian immunodeficiency virus

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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