Infection with a molecularly cloned SIVsm virus elicits high titer homologous neutralizing antibodies with heterologous neutralizing activity

M. Mahalanabis, V. M. Hirsch, Nancy L. Haigwood

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    Abstract

    We have evaluated the homologous and heterologous neutralizing antibody response in a cohort of six Macaca nemestrina infected with the cloned virus SIVsm62d that showed different levels of envelope diversification. Two progressor macaques developed AIDS by 1.5 years post-inoculation and four non-progressors were asymptomatic for 3 years of follow-up. All macaques developed high titers of neutralizing antibodies against homologous SIVsm viruses and intermediate titers against SIVsmB670. Heterologous virus neutralization of SIVmac, SIVmne, and HIV-2 was detected at much lower levels in both progressor macaques; only one of four non-progressors had evidence for broader neutralizing antibody activity. We noted changes in potential N-linked glycosylation (PNG) sites in V1/V2, C2, and V4 that were common to multiple macaques. These results support a model for viral neutralization where heterologous neutralization is, in part, driven by a strong homologous response and may be coupled to changes in PNG sites in envelop.

    Original languageEnglish (US)
    Pages (from-to)253-261
    Number of pages9
    JournalJournal of medical primatology
    Volume34
    Issue number5-6 SPEC. ISS.
    DOIs
    StatePublished - Oct 1 2005

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    Keywords

    • Broad neutralization
    • N-linked glycosylation
    • Simian immunodeficiency virus

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • veterinary(all)

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