Infection of APC by human cytomegalovirus controlled through recognition of endogenous nuclear immediate early protein 1 by specific CD4+ T lymphocytes

Emmanuelle Le Roy, Michel Baron, Wolfgang Faigle, Danièle Clément, David Lewinsohn, Daniel Streblow, Jay Nelson, Sebastian Amigorena, Jean Luc Davignon

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Infections by human CMV are controlled by cellular immune responses. Professional APC such as monocytes and macrophages can be infected in vivo and are considered as a reservoir of virus. However, CMV-specific CD4+ responses against infected APC have not been reported. To develop a model of CD4-infected APC interaction, we have transfected the U373MG astrocytoma cell line with the class II transactivator (CIITA). Confocal microscopy experiments showed that U373MG-CIITA cells expressed markers characteristic of APC. Functional assays demonstrated that infected U373MG-CIITA APC processed and presented both exogenous and endogenously neosynthesized nuclear immediate early (IE) protein 1 through the MHC class II pathway. More importantly, endogenous presentation of IE1 by infected APC lead to efficient control of CMV infection as revealed by decreased viral titer. Thus, these results describe the endogenous presentation of a nuclear viral protein by the MHC class II pathway and suggest that IE1-specific CD4+ T cells may play an important role in CMV infection by directly acting against infected APC.

Original languageEnglish (US)
Pages (from-to)1293-1301
Number of pages9
JournalJournal of Immunology
Volume169
Issue number3
StatePublished - Aug 1 2002
Externally publishedYes

Fingerprint

Immediate-Early Proteins
Nuclear Proteins
Cytomegalovirus
T-Lymphocytes
Infection
Astrocytoma
Viral Proteins
Infection Control
Confocal Microscopy
Cellular Immunity
Monocytes
Macrophages
Viruses
Cell Line
MHC class II transactivator protein

ASJC Scopus subject areas

  • Immunology

Cite this

Infection of APC by human cytomegalovirus controlled through recognition of endogenous nuclear immediate early protein 1 by specific CD4+ T lymphocytes. / Le Roy, Emmanuelle; Baron, Michel; Faigle, Wolfgang; Clément, Danièle; Lewinsohn, David; Streblow, Daniel; Nelson, Jay; Amigorena, Sebastian; Davignon, Jean Luc.

In: Journal of Immunology, Vol. 169, No. 3, 01.08.2002, p. 1293-1301.

Research output: Contribution to journalArticle

Le Roy, Emmanuelle ; Baron, Michel ; Faigle, Wolfgang ; Clément, Danièle ; Lewinsohn, David ; Streblow, Daniel ; Nelson, Jay ; Amigorena, Sebastian ; Davignon, Jean Luc. / Infection of APC by human cytomegalovirus controlled through recognition of endogenous nuclear immediate early protein 1 by specific CD4+ T lymphocytes. In: Journal of Immunology. 2002 ; Vol. 169, No. 3. pp. 1293-1301.
@article{7e429571fab842e598b69642404ccd3c,
title = "Infection of APC by human cytomegalovirus controlled through recognition of endogenous nuclear immediate early protein 1 by specific CD4+ T lymphocytes",
abstract = "Infections by human CMV are controlled by cellular immune responses. Professional APC such as monocytes and macrophages can be infected in vivo and are considered as a reservoir of virus. However, CMV-specific CD4+ responses against infected APC have not been reported. To develop a model of CD4-infected APC interaction, we have transfected the U373MG astrocytoma cell line with the class II transactivator (CIITA). Confocal microscopy experiments showed that U373MG-CIITA cells expressed markers characteristic of APC. Functional assays demonstrated that infected U373MG-CIITA APC processed and presented both exogenous and endogenously neosynthesized nuclear immediate early (IE) protein 1 through the MHC class II pathway. More importantly, endogenous presentation of IE1 by infected APC lead to efficient control of CMV infection as revealed by decreased viral titer. Thus, these results describe the endogenous presentation of a nuclear viral protein by the MHC class II pathway and suggest that IE1-specific CD4+ T cells may play an important role in CMV infection by directly acting against infected APC.",
author = "{Le Roy}, Emmanuelle and Michel Baron and Wolfgang Faigle and Dani{\`e}le Cl{\'e}ment and David Lewinsohn and Daniel Streblow and Jay Nelson and Sebastian Amigorena and Davignon, {Jean Luc}",
year = "2002",
month = "8",
day = "1",
language = "English (US)",
volume = "169",
pages = "1293--1301",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Infection of APC by human cytomegalovirus controlled through recognition of endogenous nuclear immediate early protein 1 by specific CD4+ T lymphocytes

AU - Le Roy, Emmanuelle

AU - Baron, Michel

AU - Faigle, Wolfgang

AU - Clément, Danièle

AU - Lewinsohn, David

AU - Streblow, Daniel

AU - Nelson, Jay

AU - Amigorena, Sebastian

AU - Davignon, Jean Luc

PY - 2002/8/1

Y1 - 2002/8/1

N2 - Infections by human CMV are controlled by cellular immune responses. Professional APC such as monocytes and macrophages can be infected in vivo and are considered as a reservoir of virus. However, CMV-specific CD4+ responses against infected APC have not been reported. To develop a model of CD4-infected APC interaction, we have transfected the U373MG astrocytoma cell line with the class II transactivator (CIITA). Confocal microscopy experiments showed that U373MG-CIITA cells expressed markers characteristic of APC. Functional assays demonstrated that infected U373MG-CIITA APC processed and presented both exogenous and endogenously neosynthesized nuclear immediate early (IE) protein 1 through the MHC class II pathway. More importantly, endogenous presentation of IE1 by infected APC lead to efficient control of CMV infection as revealed by decreased viral titer. Thus, these results describe the endogenous presentation of a nuclear viral protein by the MHC class II pathway and suggest that IE1-specific CD4+ T cells may play an important role in CMV infection by directly acting against infected APC.

AB - Infections by human CMV are controlled by cellular immune responses. Professional APC such as monocytes and macrophages can be infected in vivo and are considered as a reservoir of virus. However, CMV-specific CD4+ responses against infected APC have not been reported. To develop a model of CD4-infected APC interaction, we have transfected the U373MG astrocytoma cell line with the class II transactivator (CIITA). Confocal microscopy experiments showed that U373MG-CIITA cells expressed markers characteristic of APC. Functional assays demonstrated that infected U373MG-CIITA APC processed and presented both exogenous and endogenously neosynthesized nuclear immediate early (IE) protein 1 through the MHC class II pathway. More importantly, endogenous presentation of IE1 by infected APC lead to efficient control of CMV infection as revealed by decreased viral titer. Thus, these results describe the endogenous presentation of a nuclear viral protein by the MHC class II pathway and suggest that IE1-specific CD4+ T cells may play an important role in CMV infection by directly acting against infected APC.

UR - http://www.scopus.com/inward/record.url?scp=0036681794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036681794&partnerID=8YFLogxK

M3 - Article

C2 - 12133951

AN - SCOPUS:0036681794

VL - 169

SP - 1293

EP - 1301

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -