Infection of a human respiratory epithelial cell line with rhinovirus induction of cytokine release and modulation of susceptibility to infection by cytokine exposure

M. Cecilia Subauste, David Jacoby, Stephen M. Richards, David Proud

Research output: Contribution to journalArticle

323 Citations (Scopus)

Abstract

Rhinovirus infections cause over one third of all colds and are a contributing factor to exacerbations of asthma. To gain insights into the early biochemical events that occur in infected epithelial cells, we develop, for the first time, a model in which a pure respiratory epithelial cell population can be routinely infected by rhinovirus. Viral infection was confirmed by demonstrating that viral titers of supernatants and lysates from infected cell increased with time and by PCR. Infection by rhinovirus 14 was inhibited by homotypic antiserum and by antibodies to intercellular adhesion molecule-1 (ICAM-1), the receptor for this virus. Susceptibility of epithelial cells to infection by rhinovirus 14 (but not rhinovirus 2, an ICAM-1 independent strain) can be increased by preexposure of cells to TNFα, whereas IFNγ reduces susceptibility to infection by both rhinovirus strains. Rhinovirus infection per se does not markedly alter ICAM-1 expression on epithelial cells. Finally, we demonstrate that rhinovirus infection induced increased production of IL-8, IL-6, and GM-CSF from epithelial cells. Production of IL-8 correlated with viral replication during the first 24 h after infection. This model should provide useful insights into the pathogenesis of rhinovirus infections.

Original languageEnglish (US)
Pages (from-to)549-557
Number of pages9
JournalJournal of Clinical Investigation
Volume96
Issue number1
StatePublished - Jul 1995
Externally publishedYes

Fingerprint

Rhinovirus
Epithelial Cells
Cytokines
Cell Line
Infection
Intercellular Adhesion Molecule-1
Interleukin-8
Virus Receptors
Virus Diseases
Granulocyte-Macrophage Colony-Stimulating Factor
Immune Sera
Interleukin-6
Asthma
Polymerase Chain Reaction

Keywords

  • Airway
  • Asthma
  • Common cold
  • ICAM-1
  • Inflammation
  • Neutrophils

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Infection of a human respiratory epithelial cell line with rhinovirus induction of cytokine release and modulation of susceptibility to infection by cytokine exposure. / Subauste, M. Cecilia; Jacoby, David; Richards, Stephen M.; Proud, David.

In: Journal of Clinical Investigation, Vol. 96, No. 1, 07.1995, p. 549-557.

Research output: Contribution to journalArticle

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