TY - JOUR
T1 - Induction of surfactant protein A expression by cortisol facilitates prostaglandin synthesis in human chorionic trophoblasts
AU - Sun, Kang
AU - Brockman, Diane
AU - Campos, Begona
AU - Pitzer, Brad
AU - Myatt, Leslie
N1 - Funding Information:
This work was supported by NIH RO1 HD31514-10 and NSFC 30570680 and 30470655.
PY - 2006/12
Y1 - 2006/12
N2 - Context: Surfactant protein A (SP-A) may be an important link between the maturation of fetal organs and the initiation of parturition. However, the local expression of SP-A and the effect of SP-A on prostaglandin synthesis in human fetal membranes have not been resolved. Objective: Our objective was to examine SP-A expression and the effect of SP-A on prostaglandin synthesis in human fetal membranes. Design: SP-A expression was examined with immunohistochemistry and PCR. The effect of SP-A on prostaglandin synthesis was investigated in cultured human chorionic trophoblasts. Patients: Patients were normal-term pregnant women undergoing elective cesarean sections. Results: Both SP-A protein and mRNA were present in amnion epithelial cells, fibroblasts, and chorionic trophoblasts. Cortisol (10-7 and 10-6 M, 24 h) induced SP-A expression in cultured chorionic trophoblasts, which could be blocked by the glucocorticoid receptor antagonist RU486. Treatment of chorionic trophoblasts with SP-A (10-100 μg/ml, 24 h) caused a dose-dependent increase of prostaglandin E 2 release and an induction of cyclooxygenase type 2 but not cytosolic phospholipase A2 and microsomal prostaglandin E synthase expression. Conclusions: SP-A can be synthesized locally in human fetal membranes, which can be induced by glucocorticoids. SP-A appeared to induce prostaglandin E 2 synthesis in chorionic trophoblasts via induction of cyclooxygenase type 2 expression.
AB - Context: Surfactant protein A (SP-A) may be an important link between the maturation of fetal organs and the initiation of parturition. However, the local expression of SP-A and the effect of SP-A on prostaglandin synthesis in human fetal membranes have not been resolved. Objective: Our objective was to examine SP-A expression and the effect of SP-A on prostaglandin synthesis in human fetal membranes. Design: SP-A expression was examined with immunohistochemistry and PCR. The effect of SP-A on prostaglandin synthesis was investigated in cultured human chorionic trophoblasts. Patients: Patients were normal-term pregnant women undergoing elective cesarean sections. Results: Both SP-A protein and mRNA were present in amnion epithelial cells, fibroblasts, and chorionic trophoblasts. Cortisol (10-7 and 10-6 M, 24 h) induced SP-A expression in cultured chorionic trophoblasts, which could be blocked by the glucocorticoid receptor antagonist RU486. Treatment of chorionic trophoblasts with SP-A (10-100 μg/ml, 24 h) caused a dose-dependent increase of prostaglandin E 2 release and an induction of cyclooxygenase type 2 but not cytosolic phospholipase A2 and microsomal prostaglandin E synthase expression. Conclusions: SP-A can be synthesized locally in human fetal membranes, which can be induced by glucocorticoids. SP-A appeared to induce prostaglandin E 2 synthesis in chorionic trophoblasts via induction of cyclooxygenase type 2 expression.
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U2 - 10.1210/jc.2006-1472
DO - 10.1210/jc.2006-1472
M3 - Article
C2 - 17003084
AN - SCOPUS:33845511803
SN - 0021-972X
VL - 91
SP - 4988
EP - 4994
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 12
ER -