Induction of proliferation in the primate ovarian surface epithelium in vivo

Jay W. Wright, Tanja Pejovic, John Fanton, Richard Stouffer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

BACKGROUND: Epithelial ovarian cancer (EOC) is the primary ovarian malignancy affecting women. Proposed etiologies of EOC resist direct testing due to the absence of a suitable animal model, as EOC affects only primates, not other mammals. The role of proliferation in ovarian surface epithelium (OSE) transformation has been suggested but not demonstrated, nor has OSE proliferation been widely reported. We selected the rhesus macaque as a model to evaluate the unique primate OSE in vivo, and to determine whether it can undergo proliferative repair, which may relate to EOC etiology. METHODS: Macaque ovaries were collected at three stages of the cycle. Very late luteal phase ovaries were gently brushed during laparoscopy to remove a portion of the OSE, and ovaries (≤3 per group) were collected 1-4 days later. Ovary samples were also collected from 10 women aged 33-74 years. Ovarian tissue sections were probed with OSE markers (keratin, β-catenin, E- and N-cadherin), proliferation markers [proliferating cell nuclear antigen (PCNA), phosphorylated histone H3 (phospho-H3), and phosphorylated Retinoblastoma (pRb)], or labels of collagen and basement membrane. RESULTS: Brushing partially removed the OSE; did not cause tissue damage/adhesions; elevated the frequency of PCNA, phospho-H3 and pRb in the residual OSE, marking as many as 10-50% of cells in brushed regions (unbrushed areas contained

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalHuman Reproduction
Volume23
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Primates
Epithelium
Ovary
Retinoblastoma
Proliferating Cell Nuclear Antigen
Histones
Tissue Adhesions
Catenins
Luteal Phase
Macaca
Cadherins
Keratins
Macaca mulatta
Basement Membrane
Laparoscopy
Mammals
Collagen
Animal Models
Ovarian epithelial cancer
Neoplasms

Keywords

  • Cell proliferation
  • Epithelial ovarian cancer
  • Ovarian malignancy
  • Ovarian surface epithelium
  • Rhesus macaque

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Medicine(all)

Cite this

Induction of proliferation in the primate ovarian surface epithelium in vivo. / Wright, Jay W.; Pejovic, Tanja; Fanton, John; Stouffer, Richard.

In: Human Reproduction, Vol. 23, No. 1, 01.2008, p. 129-138.

Research output: Contribution to journalArticle

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