Nickel subsulfide, αNi3S2, was administered to albino Fischer rats by a single injection into the vitreous body of the right eye (0.5 mg αNi3S2/rat, suspended in 20 μl of NaCl vehicle). Control rats received a similar injection of the vehicle. Malignant tumors developed in the injected eyes of 14/15 αNiS2-treated rats by 8 months (vs. 0/11 controls, p < 0.001). Five of the injected eyes of αNi3S2 included 11 melanomas, four retinoblastomas, three gliomas, and three unclassified malignant neoplasms. Three of the melanomas developed extraocular extensions; one of the melanomas metastasized to lungs and brain. Although the melanomas arose from amelanotic uveal melanocytes, melanosomes were observed in electron micrographs of the tumor cells. This study provides a new experimental model for chemical induction of ocular neoplasms. As a procedure to test the carcinogenicity of nickel compounds, intraocular injection has the advantages of short latency period, high tumor incidence, and ease of tumor detection.
|Original language||English (US)|
|Number of pages||15|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - 1982|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience