Induction of ocular neoplasms in Fischer rats by intraocular injection of nickel subsulfide

Daniel Albert, J. R. Gonder, J. Papale, J. L. Craft, H. G. Dohlman, M. C. Reid, F. W. Sunderman

Research output: Contribution to journalArticle

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Abstract

Nickel subsulfide, αNi3S2, was administered to albino Fischer rats by a single injection into the vitreous body of the right eye (0.5 mg αNi3S2/rat, suspended in 20 μl of NaCl vehicle). Control rats received a similar injection of the vehicle. Malignant tumors developed in the injected eyes of 14/15 αNiS2-treated rats by 8 months (vs. 0/11 controls, p < 0.001). Five of the injected eyes of αNi3S2 included 11 melanomas, four retinoblastomas, three gliomas, and three unclassified malignant neoplasms. Three of the melanomas developed extraocular extensions; one of the melanomas metastasized to lungs and brain. Although the melanomas arose from amelanotic uveal melanocytes, melanosomes were observed in electron micrographs of the tumor cells. This study provides a new experimental model for chemical induction of ocular neoplasms. As a procedure to test the carcinogenicity of nickel compounds, intraocular injection has the advantages of short latency period, high tumor incidence, and ease of tumor detection.

Original languageEnglish (US)
Pages (from-to)768-782
Number of pages15
JournalInvestigative Ophthalmology and Visual Science
Volume22
Issue number6
StatePublished - Oct 25 1982
Externally publishedYes

Fingerprint

nickel subsulfide
Eye Neoplasms
Intraocular Injections
Inbred F344 Rats
Melanoma
Neoplasms
Carcinogenicity Tests
Amelanotic Melanoma
Melanosomes
Vitreous Body
Injections
Retinoblastoma
Melanocytes
Nickel
Glioma
Theoretical Models
Electrons
Lung

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Albert, D., Gonder, J. R., Papale, J., Craft, J. L., Dohlman, H. G., Reid, M. C., & Sunderman, F. W. (1982). Induction of ocular neoplasms in Fischer rats by intraocular injection of nickel subsulfide. Investigative Ophthalmology and Visual Science, 22(6), 768-782.

Induction of ocular neoplasms in Fischer rats by intraocular injection of nickel subsulfide. / Albert, Daniel; Gonder, J. R.; Papale, J.; Craft, J. L.; Dohlman, H. G.; Reid, M. C.; Sunderman, F. W.

In: Investigative Ophthalmology and Visual Science, Vol. 22, No. 6, 25.10.1982, p. 768-782.

Research output: Contribution to journalArticle

Albert, D, Gonder, JR, Papale, J, Craft, JL, Dohlman, HG, Reid, MC & Sunderman, FW 1982, 'Induction of ocular neoplasms in Fischer rats by intraocular injection of nickel subsulfide', Investigative Ophthalmology and Visual Science, vol. 22, no. 6, pp. 768-782.
Albert, Daniel ; Gonder, J. R. ; Papale, J. ; Craft, J. L. ; Dohlman, H. G. ; Reid, M. C. ; Sunderman, F. W. / Induction of ocular neoplasms in Fischer rats by intraocular injection of nickel subsulfide. In: Investigative Ophthalmology and Visual Science. 1982 ; Vol. 22, No. 6. pp. 768-782.
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