Induction of ocular neoplasms in Fischer rats by intraocular injection of nickel subsulfide

D. M. Albert, J. R. Gonder, J. Papale, J. L. Craft, H. G. Dohlman, M. C. Reid, F. W. Sunderman

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Nickel subsulfide, αNi3S2, was administered to albino Fischer rats by a single injection into the vitreous body of the right eye (0.5 mg αNi3S2/rat, suspended in 20 μl of NaCl vehicle). Control rats received a similar injection of the vehicle. Malignant tumors developed in the injected eyes of 14/15 αNiS2-treated rats by 8 months (vs. 0/11 controls, p < 0.001). Five of the injected eyes of αNi3S2 included 11 melanomas, four retinoblastomas, three gliomas, and three unclassified malignant neoplasms. Three of the melanomas developed extraocular extensions; one of the melanomas metastasized to lungs and brain. Although the melanomas arose from amelanotic uveal melanocytes, melanosomes were observed in electron micrographs of the tumor cells. This study provides a new experimental model for chemical induction of ocular neoplasms. As a procedure to test the carcinogenicity of nickel compounds, intraocular injection has the advantages of short latency period, high tumor incidence, and ease of tumor detection.

Original languageEnglish (US)
Pages (from-to)768-782
Number of pages15
JournalInvestigative Ophthalmology and Visual Science
Issue number6
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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