TY - JOUR
T1 - Induction of ocular neoplasms in Fischer rats by intraocular injection of nickel subsulfide
AU - Albert, D. M.
AU - Gonder, J. R.
AU - Papale, J.
AU - Craft, J. L.
AU - Dohlman, H. G.
AU - Reid, M. C.
AU - Sunderman, F. W.
PY - 1982/10/25
Y1 - 1982/10/25
N2 - Nickel subsulfide, αNi3S2, was administered to albino Fischer rats by a single injection into the vitreous body of the right eye (0.5 mg αNi3S2/rat, suspended in 20 μl of NaCl vehicle). Control rats received a similar injection of the vehicle. Malignant tumors developed in the injected eyes of 14/15 αNiS2-treated rats by 8 months (vs. 0/11 controls, p < 0.001). Five of the injected eyes of αNi3S2 included 11 melanomas, four retinoblastomas, three gliomas, and three unclassified malignant neoplasms. Three of the melanomas developed extraocular extensions; one of the melanomas metastasized to lungs and brain. Although the melanomas arose from amelanotic uveal melanocytes, melanosomes were observed in electron micrographs of the tumor cells. This study provides a new experimental model for chemical induction of ocular neoplasms. As a procedure to test the carcinogenicity of nickel compounds, intraocular injection has the advantages of short latency period, high tumor incidence, and ease of tumor detection.
AB - Nickel subsulfide, αNi3S2, was administered to albino Fischer rats by a single injection into the vitreous body of the right eye (0.5 mg αNi3S2/rat, suspended in 20 μl of NaCl vehicle). Control rats received a similar injection of the vehicle. Malignant tumors developed in the injected eyes of 14/15 αNiS2-treated rats by 8 months (vs. 0/11 controls, p < 0.001). Five of the injected eyes of αNi3S2 included 11 melanomas, four retinoblastomas, three gliomas, and three unclassified malignant neoplasms. Three of the melanomas developed extraocular extensions; one of the melanomas metastasized to lungs and brain. Although the melanomas arose from amelanotic uveal melanocytes, melanosomes were observed in electron micrographs of the tumor cells. This study provides a new experimental model for chemical induction of ocular neoplasms. As a procedure to test the carcinogenicity of nickel compounds, intraocular injection has the advantages of short latency period, high tumor incidence, and ease of tumor detection.
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M3 - Article
C2 - 7076422
AN - SCOPUS:0019983028
VL - 22
SP - 768
EP - 782
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 6
ER -